重新检验被报道为产A型单端孢霉烯族化合物的禾谷镰刀菌 NRRL-13820 和 NRRL-13852。

Reexamination ofFusarium graminearum NRRL-13820and NRRL-13852 reported as type A trichothecene producers.

机构信息

Istituto tossine e micotossine da parassiti vegetali, CNR, Viale Einaudi 51, 70125, Bari, Italy.

出版信息

Mycotoxin Res. 1993 Mar;9(1):41-6. doi: 10.1007/BF03192231.

DOI:10.1007/BF03192231

Abstract

NRRL-13820 and NRRL-13852 are reported to be two atypicalFusarium graminearum strains type A trichothecene producers [T-2 toxin (T-2) and diacetoxy-scirpenol (DAS)]. These two strains were reexamined by morphological, genetical (DNA / DNA relatedness) and toxicological techniques and compared with 28 wildF graminearum isolates obtained from corn in Italy and the USA. The isolate NRRL-13820 was morphologically confirmed as a typical isolate ofF graminearum, while the isolate NRRL-13852 showed some peculiar characteristics. Nuclear DNA comparison between NRRL-13820 and NRRL-13852 displayed 49% similarity and showed 94 % and 44 % relatedness, respectively, when compared withF graminearum NRRL-13833, which is a well assessed type B trichothecene producer [deoxynivalenol (DON) and 15-acetyldeoxynivalenol]. NRRL-13820, NRRL-13852, and NRRL-13833, as well as the 28 wild isolates, were not able to synthesize T-2, HT-2 nor DAS. Finally, NRRL-13820 and NRRL-13833, but not NRRL-13852, were able to produce DON (120 and 40/μg/g, respectively). The data support the concept that the production of examined type A trichothecenes is very rare inF graminearum.

摘要

NRRL-13820 和 NRRL-13852 被报道为两种非典型的禾谷镰刀菌 A 型产毒菌株，能够产生 T-2 毒素（T-2）和二乙酰镳草镰刀菌醇（DAS）。这两种菌株通过形态学、遗传学（DNA/DNA 同源性）和毒理学技术进行了重新检查，并与从意大利和美国玉米中获得的 28 株野生禾谷镰刀菌分离株进行了比较。分离株 NRRL-13820 在形态上被确认为典型的禾谷镰刀菌分离株，而分离株 NRRL-13852 则表现出一些特殊的特征。NRRL-13820 和 NRRL-13852 之间的核 DNA 比较显示相似度为 49%，与已知的 B 型产毒菌株 NRRL-13833（产脱氧雪腐镰刀菌烯醇（DON）和 15-乙酰脱氧雪腐镰刀菌烯醇）相比，相似度分别为 94%和 44%。NRRL-13820、NRRL-13852 和 NRRL-13833 以及 28 株野生分离株均不能合成 T-2、HT-2 或 DAS。最后，NRRL-13820 和 NRRL-13833 能够产生 DON（分别为 120 和 40μg/g），但 NRRL-13852 不能。这些数据支持了这样一种概念，即 examined 型 A 类镰刀菌毒素的产生在禾谷镰刀菌中非常罕见。