免疫亲和柱净化-高效液相色谱法测定小麦和猪肝中的赭曲霉毒素 A。
Determination of ochratoxin A by immunoaffinity column clean-up and HPLC In wheat and pig liver.
机构信息
Rhône Poulenc Diagnostics West of Scotland Science Park 3.06 Kelvin, Campus Maryhill Road, G20 OSP, Glasgow, Scotland.
出版信息
Mycotoxin Res. 1995 Sep;11(2):111-6. doi: 10.1007/BF03192073.
Determination of ochratoxin A (OTA) by immunoaffinity column clean-up and HPLC detection was performed on wheat and pig liver. Several extraction protocols involving methanol and ethyl acetate were investigated. The optimum experimental conditions for analysis of OTA in artificially contaminated wheat (87.4% recovery) using immunoaffinity column clean-up was found to be the methanol: PBS (1∶1 v/v) protocol. These conditions, however, gave low recoveries for pig liver (40.4%).
采用免疫亲和柱净化和高效液相色谱法检测小麦和猪肝中的赭曲霉毒素 A（OTA）。研究了几种涉及甲醇和乙酸乙酯的提取方案。使用免疫亲和柱净化分析人工污染小麦中 OTA 的最佳实验条件（回收率为 87.4%）是甲醇：PBS（1∶1 v/v）方案。然而，这些条件对猪肝的回收率较低（40.4%）。
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