Dunne C, Meaney M, Smyth M, Tuinstra L G
School of Chemical Sciences, Dublin City University, Glasnevin, Ireland.
J Chromatogr. 1993 Jan 22;629(2):229-35. doi: 10.1016/0021-9673(93)87036-l.
A sensitive and reliable method is described for the determination of aflatoxins B1, B2, G1 and G2, ochratoxin A and zearalenone in animal feed ingredients. A multi-toxin extraction and clean-up procedure is used, with dichloromethane-1 M hydrochloric acid (10:1) being used for the extraction and gel permeation chromatography being used for the clean-up. The liquid chromatographic method developed for the separation of the six mycotoxins involves gradient elution with a reversed-phase C18 column and fluorescence detection. Recoveries, repeatability and reproducibility have been determined on maize, palm and wheat. The detection limits varied depending on the type of feed.
描述了一种灵敏且可靠的方法，用于测定动物饲料原料中的黄曲霉毒素B1、B2、G1和G2、赭曲霉毒素A以及玉米赤霉烯酮。采用了多毒素提取和净化程序，用二氯甲烷-1M盐酸（10:1）进行提取，用凝胶渗透色谱法进行净化。为分离这六种霉菌毒素开发的液相色谱方法涉及使用反相C18柱进行梯度洗脱和荧光检测。已在玉米、棕榈和小麦上测定了回收率、重复性和再现性。检测限因饲料类型而异。
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