Laharrague P, Corberand J X, Fillola G, Marcelino N
Laboratoire Central d'Hématologie, CHU Rangueil, Toulouse.
Ann Biol Clin (Paris). 1990;48(4):253-8.
The microscopic enumeration of reticulocytes is a tedious assay with poor reproducibility. Its results provide only fragmentory information on the kinetics of erythropoiesis. Thus, attempts have been made for its automation. This study presents results of an evaluation of the first machine exclusively devoted to reticulocyte analysis. The Sysmex R-1 000 uses flow cytofluorometry with argon laser, after precipitation of nucleic acids by auramine-O. The whole study was performed according to ICSH recommendations. At least 2,500 erythrocytes were examined in the microscopic reference technique, after 1 p. cent brilliant cresyl-blue staining. Within and between-batch precision studies gave CV not exceeding 5.46 and 8.72 p. cent respectively for reticulocyte numbers. In dilution study it was shown that no measured result was over 4 p. cent of the expected value, in the range 4-400 x 10(9)/l. There was no significant carry-over in the range tested (35-510 x 10(9)/l). Accuracy testing proved that the significant difference observed when microscopic enumeration was performed in routine conditions disappeared when at least 5,000 erythrocytes were examined. Keeping the samples at 4 degrees C limited the decrease of the reticulocyte number to less than 10 p. cent after 24 hours. Analysis of some particular clinical situations demonstrated the risk of spurious results in case of massive Plasmodium infestation, and the usefulness of obtaining the percentage of newly emerged reticulocytes, i.e. the most fluorescent, for a better estimate of the kinetics of erythrocyte production. This evaluation testifies the quality of the Sysmex R-1 000, and underlines the potential benefit of such analyzers in the equipment of the modern hematology laboratory.
网织红细胞的显微镜计数是一项繁琐且重复性差的检测方法。其结果仅能提供有关红细胞生成动力学的零碎信息。因此，人们尝试对其进行自动化操作。本研究展示了对第一台专门用于网织红细胞分析的仪器的评估结果。Sysmex R - 1000在使用金胺 - O沉淀核酸后，采用氩离子激光流式细胞荧光术。整个研究是按照国际血液学标准化委员会（ICSH）的建议进行的。在显微镜参考技术中，经1%煌焦油蓝染色后，至少检查2500个红细胞。批内和批间精密度研究表明，网织红细胞计数的变异系数（CV）分别不超过5.46%和8.72%。在稀释研究中发现，在4 - 400×10⁹/L范围内，没有测量结果超过预期值的4%。在所测试的范围（35 - 510×10⁹/L）内没有明显的残留。准确性测试证明，在常规条件下进行显微镜计数时观察到的显著差异，在检查至少5000个红细胞时消失。将样本保存在4℃下，24小时后网织红细胞数量的减少限制在10%以内。对一些特殊临床情况的分析表明，在大量疟原虫感染的情况下存在假结果的风险，以及获取新出现的网织红细胞（即荧光最强的网织红细胞）百分比对于更好地评估红细胞生成动力学的有用性。这项评估证明了Sysmex R - 1000的质量，并强调了此类分析仪在现代血液学实验室设备中的潜在益处。
