Department of Pharmacology, Hanagal Shri Kumareshwar College of Pharmacy, B.V.V.S Campus, Bagalkot, Karnataka, India.
Pharm Biol. 2013 Aug;51(8):955-60. doi: 10.3109/13880209.2013.771685. Epub 2013 Apr 22.
Stereospermum suaveolens DC. (Bignoniaceae) is a medicinal tree species native to India. Traditionally, the whole plant is used for various diseases including neuronal disorders.
The present study evaluated the neuroprotective activity of Stereospermum suaveolens against global cerebral ischemia in a rat model.
Neuroprotective activity was carried out by global cerebral ischemia on Sprague-Dawley rats and divided into five groups of eight rats each; sham and control groups received normal saline (10 ml/kg) and treated groups received methanol extract of Stereospermum suaveolens (MES) orally (125, 250, and 500 mg/kg) for 10 days prior to the experiment. Global cerebral ischemia was induced by bilateral carotid artery (BCA) occlusion for 30 min followed by 4-h reperfusion. The antioxidant enzymatic and non-enzymatic levels were estimated by UV spectroscopic method along with cerebral infarction area; histopathological studies were carried out.
LD₅₀ of MES was found to be 5000 mg/kg of body weight. The entire test was performed at dose levels 125, 250, and 500 mg/kg of body weight. The results of the study indicate that the Stereospermum suaveolens methanol extract showed neuroprotective activity by a significant decrease in lipid peroxidation (p < 0.001) and an increase in superoxide dismutase (p < 0.01), catalase (p < 0.01), glutathione (p < 0.001), and total thiol (p < 0.001) levels in extract-treated groups as compared to control group. Measurement of cerebral infarction area and histopathological studies further supported the protective effect of the extract.
These findings suggest a potential protective role of Stereospermum suaveolens against global cerebral ischemia/reperfusion-induced brain injury.
Stereospermum suaveolens DC.（夹竹桃科）是一种原产于印度的药用树木。传统上，整株植物被用于治疗各种疾病，包括神经紊乱。
本研究评估了 Stereospermum suaveolens 对大鼠全脑缺血模型的神经保护活性。
通过 Sprague-Dawley 大鼠的全脑缺血进行神经保护活性实验，将大鼠分为五组，每组 8 只；假手术组和对照组给予生理盐水（10ml/kg），治疗组给予 Stereospermum suaveolens 甲醇提取物（MES）口服（125、250 和 500mg/kg），在实验前 10 天进行。通过双侧颈总动脉（BCA）闭塞 30min 后再灌注 4h 诱导全脑缺血。通过紫外分光光度法估计抗氧化酶和非酶水平，并评估脑梗死面积；进行组织病理学研究。
MES 的 LD₅₀ 为 5000mg/kg 体重。整个实验在 125、250 和 500mg/kg 体重剂量水平下进行。研究结果表明，Stereospermum suaveolens 甲醇提取物通过显著降低脂质过氧化（p<0.001）和增加超氧化物歧化酶（p<0.01）、过氧化氢酶（p<0.01）、谷胱甘肽（p<0.001）和总巯基（p<0.001）水平，显示出神经保护活性，与对照组相比，提取物处理组的水平有所增加。脑梗死面积测量和组织病理学研究进一步支持了提取物的保护作用。
这些发现表明，Stereospermum suaveolens 对全脑缺血/再灌注诱导的脑损伤具有潜在的保护作用。
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