Department of Pharmacology, Hanagal Shri Kumareshwar College of Pharmacy, B.V.V.S Campus, Bagalkot, Karnataka, India.
Department of Pharmacology, Hanagal Shri Kumareshwar College of Pharmacy, B.V.V.S Campus, Bagalkot, Karnataka, India.
J Ethnopharmacol. 2020 Mar 1;249:112393. doi: 10.1016/j.jep.2019.112393. Epub 2019 Nov 16.
Convolvulus pluricaulis Choisy commonly known as Shankhapushpi, is traditionally prescribed for nerve debility, loss of memory, epilepsy and as nervine tonic. Plant also proved to have diverse pharmacological activity but the neuroprotection in ischemic stroke were not found.
To investigate the effect of Convolvulus pluricaulis against bilateral common carotid artery (BCCA) occlusion induced cerebral ischemic reperfusion injury.
The neuroprotective activity of Convolvulus pluricaulis against bilateral common carotid artery (BCCA) occlusion induced cerebral ischemic reperfusion (I/S) injury. Sprague-Dawley rats of either sex (200-250 g) were divided into nine groups of 8 rats each. Sham and control group, saline treated 10 ml/kg orally. Third group treated with Quercetin 25 mg/kg orally and fourth to ninth groups treated with chloroform and ethanol extract of Convolvulus pluricaulis 100, 200, and 400 mg/kg (p.o.) respectively. Control, Quercetin and extract treated groups underwent 30 min BCCA occlusion and 24 h reperfusion on 10th day but sham underwent same surgery without BCCA occlusion and 24 h reperfusion on 10th day. The antioxidant enzymatic and non-enzymatic levels were estimated by UV spectroscopic method and cerebral infarction area, Blood brain barrier disruption, microtubule-associated protein 2 immunohistochemical and histopathological studies were carried out.
The results of the study indicate that the chloroform and ethanol extract of Convolvulus pluricaulis showed neuroprotective activity by a significant decrease in lipid peroxidation (p < 0.001) and an increase in superoxide dismutase (p < 0.01, p < 0.001), catalase (p < 0.01, p < 0.001), glutathione (p < 0.001), and total thiol (p < 0.001) levels in extract-treated groups as compared to control group. Measurement of cerebral infarction area, blood brain barrier disruption, microtubule-associated protein 2 immunohistochemical and histopathological studies further supported the protective effect of the extract.
Present study revile that Convolvulus pluricaulis has potent neuroprotection against bilateral common carotid artery (BCCA) occlusion induced cerebral ischemic reperfusion injury.
旋花属植物俗称 Shankhapushpi，传统上用于治疗神经虚弱、记忆力减退、癫痫和神经滋补剂。该植物已被证明具有多种药理学活性，但在缺血性中风中没有发现神经保护作用。
研究旋花属植物对双侧颈总动脉（BCCA）闭塞诱导的脑缺血再灌注损伤的影响。
旋花属植物对双侧颈总动脉（BCCA）闭塞诱导的脑缺血再灌注（I/S）损伤的神经保护活性。将雄性和雌性斯普拉格-道利大鼠（200-250g）分为 9 组，每组 8 只。假手术组和对照组给予生理盐水 10ml/kg 灌胃。第三组给予槲皮素 25mg/kg 灌胃，第四至第九组分别给予旋花属植物的氯仿和乙醇提取物 100、200 和 400mg/kg（p.o.）。对照组、槲皮素和提取物处理组在第 10 天进行 30 分钟的 BCCA 闭塞和 24 小时再灌注，但假手术组在第 10 天进行相同的手术而不进行 BCCA 闭塞和 24 小时再灌注。通过紫外分光光度法估计抗氧化酶和非酶水平，并进行脑梗死面积、血脑屏障破坏、微管相关蛋白 2 免疫组织化学和组织病理学研究。
研究结果表明，旋花属植物的氯仿和乙醇提取物通过显著降低脂质过氧化（p<0.001）和增加超氧化物歧化酶（p<0.01，p<0.001）、过氧化氢酶（p<0.01，p<0.001）、谷胱甘肽（p<0.001）和总巯基（p<0.001）水平，表现出神经保护活性。与对照组相比，在提取物处理组中。脑梗死面积、血脑屏障破坏、微管相关蛋白 2 免疫组织化学和组织病理学研究的测量进一步支持了提取物的保护作用。
本研究表明，旋花属植物对双侧颈总动脉（BCCA）闭塞诱导的脑缺血再灌注损伤具有潜在的神经保护作用。
