Fight against Angiogenesis-Related Blindness (FARB) Laboratory, Clinical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea; Department of Ophthalmology, College of Medicine, Seoul National University, Seoul, Republic of Korea.
Acta Histochem. 2013 Oct;115(8):789-94. doi: 10.1016/j.acthis.2013.03.005. Epub 2013 Apr 19.
The primary cilia are considered as "cellular antennae" that sense and interchange information with the extracellular environment. Nearly all mammalian cells have a single primary cilium. In the retina, the outer segment of the photoreceptor is known to be a specialized form of primary cilium, but studies on cilia in other layers of the retina are scarce. In this study, we investigated the expression of primary cilia in the whole thickness of the mouse retina using immunofluorescence with three different ciliary markers: Arl13b, acetylated α-tubulin and adenylyl cyclase III. Our results show positive reactions in the photoreceptor layer, outer plexiform layer and ganglion cell layer, which might suggest the possible presence of primary cilia in these areas, but we could not directly prove the strand-like shape of cilia in those areas. In the outer plexiform layer, all three markers showed intense staining along the neuronal synapses, which suggests that the neuronal processes themselves might share the features of cilia.
初级纤毛被认为是“细胞天线”，可感知细胞外环境并与之进行信息交换。几乎所有哺乳动物细胞都有一个初级纤毛。在视网膜中，感光器的外节被认为是初级纤毛的一种特殊形式，但对视网膜其他层的纤毛研究却很少。在这项研究中，我们使用三种不同的纤毛标记物（Arl13b、乙酰化α-微管蛋白和腺苷酸环化酶 III）通过免疫荧光法研究了小鼠整个视网膜厚度中初级纤毛的表达。我们的结果显示在感光器层、外丛状层和节细胞层中均有阳性反应，这可能表明这些区域可能存在初级纤毛，但我们无法直接证明这些区域纤毛的线状形态。在外丛状层中，所有三种标记物均沿着神经元突触强烈染色，这表明神经元突起本身可能具有纤毛的特征。
