Department of Immunology, Kyoto Prefectural University of Medicine, Kamikyo, Kyoto, 602-8566, Japan.
Biotechnol Lett. 2013 Aug;35(8):1297-301. doi: 10.1007/s10529-013-1204-8. Epub 2013 Apr 23.
Some viruses are sensitive to high pressure. The freeze-pressure generation method (FPGM) applies pressure as high as 250 MPa on a substance, simply by freezing a pressure-resistant reservoir in which the substance is immersed in water. Here we examined whether the FPGM successfully inactivates herpes simplex virus type 1 (HSV-1), an enveloped DNA virus belonging to the human Herpesviridae, and encephalomyocarditis virus (EMCV), an envelope-free RNA virus belonging to the Picornaviridae. After the treatment, HSV-1 drastically reduced the ability to form plaque in Vero cells in vitro as well as to kill mice in vivo. EMCV that had been pressurized failed to proliferate in HeLa cells and induce interferon response. The results suggest that the FPGM provides a feasible procedure to inactivate a broad spectrum of viruses.
有些病毒对高压敏感。冷冻压力产生法（FPGM）通过将耐压储液器冷冻在水中来对物质施加高达 250 MPa 的压力。在这里，我们研究了 FPGM 是否成功地灭活了单纯疱疹病毒 1 型（HSV-1），这是一种属于人类疱疹病毒科的包膜 DNA 病毒，以及脑炎心肌炎病毒（EMCV），这是一种无包膜的 RNA 病毒，属于小 RNA 病毒科。处理后，HSV-1 在体外的 Vero 细胞中形成斑的能力以及在体内杀死小鼠的能力大大降低。加压的 EMCV 未能在 HeLa 细胞中增殖并诱导干扰素反应。结果表明，FPGM 为灭活广谱病毒提供了一种可行的方法。
