Nakagami T, Shigehisa T, Ohmori T, Taji S, Hase A, Kimura T, Yamanishi K
Laboratory for Bio-Resource Science, Nippon Meat Packers, Inc., Ibaraki, Japan.
J Virol Methods. 1992 Aug;38(2):255-61. doi: 10.1016/0166-0934(92)90115-t.
The effects of high hydrostatic pressure on herpes simplex virus type 1 (HSV-1) and human cytomegalovirus (HCMV) were examined. Pressure at more than 300 MPa for 10 min at 25 degrees C inactivated these virions and drastically inhibited their infection to cultured cells, and at greater than 400 MPa, reduced infective titers of HSV-1 and HCMV by more than 7 and 4 logs, respectively. Electron microscopic examination illustrated coincidentally that high pressure at 300 MPa damaged the virus envelope and prevented the virus particles from binding to the cells. The findings suggest that treatment at high hydrostatic pressure is promising as a means of inactivating HSV-1, HCMV and other enveloped viruses.
研究了高静水压对单纯疱疹病毒1型（HSV-1）和人巨细胞病毒（HCMV）的影响。在25℃下，300MPa以上的压力处理10分钟可使这些病毒粒子失活，并显著抑制其对培养细胞的感染，而在大于400MPa时，HSV-1和HCMV的感染滴度分别降低超过7个对数和4个对数。电子显微镜检查同时表明，300MPa的高压破坏了病毒包膜，阻止病毒粒子与细胞结合。这些发现表明，高静水压处理有望成为一种灭活HSV-1、HCMV和其他包膜病毒的方法。
