Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, People's Republic of China.
Macromol Biosci. 2013 Jun;13(6):755-66. doi: 10.1002/mabi.201200470. Epub 2013 Apr 22.
A combined sulfated silk fibroin scaffold is fabricated by modifying a knitted silk scaffold with sulfated silk fibroin sponges. In vitro hemocompatibility evaluation reveals that the combined sulfated silk fibroin scaffolds reduce platelet adhesion and activation, and prolong the activated partial thromboplastin time (APTT), thrombin time (TT), and prothrombin time (PT). The response of porcine endothelial cells (ECs) and smooth muscle cells (SMCs) on the scaffolds is studied to evaluate the cytocompatibility of the scaffolds. Vascular cells are seeded on the scaffolds and cultured for 2 weeks. The scaffolds demonstrate enhanced EC adhesion, proliferation, and maintenance of cellular functions. Moreover, the scaffolds inhibit SMC proliferation and induce expression of contractile SMC marker genes.
一种复合硫酸化丝素纤维支架是通过修饰带有硫酸化丝素纤维海绵的针织丝素支架而制备的。体外血液相容性评价表明，复合硫酸化丝素纤维支架可减少血小板黏附和激活，并延长活化部分凝血活酶时间（APTT）、凝血酶时间（TT）和凝血酶原时间（PT）。研究了支架上猪内皮细胞（EC）和平滑肌细胞（SMC）的反应，以评估支架的细胞相容性。将血管细胞接种到支架上并培养 2 周。支架显示出增强的 EC 黏附、增殖和细胞功能的维持。此外，支架抑制 SMC 增殖并诱导收缩性 SMC 标记基因的表达。
