Department of Chemistry and Institute of Biomedical Science, Fudan University, 220 Handan Road, Shanghai, 200433, China.
Angew Chem Int Ed Engl. 2013 Jun 3;52(23):6055-8. doi: 10.1002/anie.201300566. Epub 2013 Apr 22.
An aptamer-based strategy was developed for the high-throughput analysis of protein biomarkers, such as lysozyme, by on-target MALDI-TOF MS. The aptamers were immobilized on the target plate through formation of covalent bonds with a stable and porous gold layer. An infrared laser was subsequently applied for fast proteolysis. High sensitivities were observed both in standard solutions and human urine.
建立了一种基于适体的策略，通过靶标 MALDI-TOF MS 对溶菌酶等蛋白质生物标志物进行高通量分析。适体通过与稳定多孔的金层形成共价键固定在靶板上。随后应用红外激光进行快速蛋白水解。在标准溶液和人尿中均观察到高灵敏度。
