Jousselin-Hosaja M, Mailly P, Tsuji S
Université P. et M. Curie, Département de Neurobiologie des Signaux Intercellulaires, CNRS URA 1488, Paris, France.
Cell Tissue Res. 1993 Oct;274(1):199-205. doi: 10.1007/BF00328001.
The development of neuron-like cholinergic immunophenotypes by adrenal chromaffin cells was studied in 10-week-old mouse adrenal medullary grafts. Fragments of chromaffin tissue were implanted into mouse hippocampus, and antibodies specific for neurofilaments (NF), neuron-specific enolase (NSE), choline acetyltransferase (ChAT), acetylcholinesterase (AChE), and phenylethanolamine-N-methyltransferase (PNMT) were applied to the grafts. Adrenal medulla grafts survived well and most of the transplanted cells were either round or polygonal. A minority of chromaffin cells elaborated an intermediate or sympathetic neuron phenotype. Chromaffin cells showed pronounced immunoreactivity for NSE in their perikarya and axon-like processes: immunoreactivity for NF was only found in a few processes. In adjacent immunohistochemically stained sections, the transplanted cells stained for ChAT and AChE. At the electron-microscope level, the immunohistochemical reactions for the two acetylcholine-related enzymes were mainly located on the endoplasmic reticulum and in cell processes. Immunoreactivity for PNMT was found to decline in transplanted chromaffin cells below that of normal adrenal medulla. These observations suggest that, in adrenal medullary grafts implanted into the hippocampus, chromaffin cells are endowed with neuron-like cholinergic immunophenotypes.
在10周龄小鼠的肾上腺髓质移植体中，研究了肾上腺嗜铬细胞向神经元样胆碱能免疫表型的发育情况。将嗜铬组织碎片植入小鼠海马体，并将针对神经丝（NF）、神经元特异性烯醇化酶（NSE）、胆碱乙酰转移酶（ChAT）、乙酰胆碱酯酶（AChE）和苯乙醇胺 - N - 甲基转移酶（PNMT）的抗体应用于移植体。肾上腺髓质移植体存活良好，大多数移植细胞呈圆形或多边形。少数嗜铬细胞呈现出中间型或交感神经元表型。嗜铬细胞在其胞体和轴突样突起中对NSE表现出明显的免疫反应性：仅在少数突起中发现对NF的免疫反应性。在相邻的免疫组织化学染色切片中，移植细胞对ChAT和AChE染色。在电子显微镜水平上，两种与乙酰胆碱相关酶的免疫组织化学反应主要位于内质网和细胞突起中。发现移植的嗜铬细胞中PNMT的免疫反应性低于正常肾上腺髓质。这些观察结果表明，在植入海马体的肾上腺髓质移植体中，嗜铬细胞具有神经元样胆碱能免疫表型。
