Takeuchi T, Matsushima T, Sugimura T
Clin Chim Acta. 1975 Apr 16;60(2):207-13. doi: 10.1016/0009-8981(75)90127-8.
Human alpha-amylase (alpha-1,4-glucan-4-glucanohydrolase, EC 3.2.1.1) was separated by electrofocusing. The amylases in serum and urine were seperated into two major isozymes with isoelectric points of pH 6.4-6.5 and pH 6.9-7.0, respectively, and one minor isozyme with an isoelectric point of pH 5.9. The amylases in saliva were separated into one major isozyme with an isoelectric point of pH 6.5 and two minor isozymes with isoelectric points of pH 6.0 and 6.9. The amylases in pancreatic juice gave one major peak at pH 7.0 and two minor peaks at pH 6.0 and pH 6.5. Repeated electrofocusing of each isozyme have only a single peak with a constant isoelectric point. The antigenicites of these three isozymes were also investigated using antisera to rat pancreatic and parotid amylases.
人α-淀粉酶（α-1,4-葡聚糖-4-葡聚糖水解酶，EC 3.2.1.1）通过等电聚焦进行分离。血清和尿液中的淀粉酶被分离为两种主要同工酶，其等电点分别为pH 6.4 - 6.5和pH 6.9 - 7.0，以及一种等电点为pH 5.9的次要同工酶。唾液中的淀粉酶被分离为一种等电点为pH 6.5的主要同工酶和两种等电点为pH 6.0和pH 6.9的次要同工酶。胰液中的淀粉酶在pH 7.0处出现一个主峰，在pH 6.0和pH 6.5处出现两个次峰。对每种同工酶进行重复等电聚焦，均只有一个具有恒定等电点的峰。还使用针对大鼠胰腺和腮腺淀粉酶的抗血清研究了这三种同工酶的抗原性。
