大鼠胰腺和腮腺淀粉酶的电泳迁移率及免疫原性的比较研究

Comparative studies on electrophoretic mobility and immunogenicity of pancreatic and parotid amylases of rat.

Takeuchi T, Mura M, Sasaki R, Matsushima T, Sugimura T

Biochim Biophys Acta. 1975 Oct 22;403(2):456-60. doi: 10.1016/0005-2744(75)90073-x.

Abstract

The alpha-amylases (1,4-alpha-D-glucan glucanohydrolase, EC 3.2.1.1) of rat serum, urine, pancreas, parotid gland and liver were separated by electrophoresis on a cellulose acetate membrane. They were found to be of three different types: a parotid gland type, a pancreatic type and a liver type. Rat serum and urine contained parotid type amylase only. 2. Antisera were prepared in rabbits against purified rat pancreatic amylase and parotid amylase. In addition to strong reactions between pancreatic amylase and its antiserum and between parotid amylase and its antiserum, a weak cross-reaction was observed between parotid amylase and anti-pancreatic amylaseserum. Anti-parotid-amylase serum gave an immunoprecipitation line with rat serum and urine, but anti-pancreatic-amylase serum did not, indicating that the amylases in serum and urine originate from parotid amylase.

摘要

采用醋酸纤维素膜电泳法对大鼠血清、尿液、胰腺、腮腺和肝脏中的α-淀粉酶（1,4-α-D-葡聚糖葡聚糖水解酶，EC 3.2.1.1）进行分离。结果发现它们有三种不同类型：腮腺型、胰腺型和肝脏型。大鼠血清和尿液中仅含有腮腺型淀粉酶。2. 用纯化的大鼠胰腺淀粉酶和腮腺淀粉酶对家兔制备抗血清。除了胰腺淀粉酶与其抗血清之间以及腮腺淀粉酶与其抗血清之间有强烈反应外，还观察到腮腺淀粉酶与抗胰腺淀粉酶血清之间有微弱的交叉反应。抗腮腺淀粉酶血清与大鼠血清和尿液产生免疫沉淀线，但抗胰腺淀粉酶血清则不产生，这表明血清和尿液中的淀粉酶源自腮腺淀粉酶。