Botnar Research Centre, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, University of Oxford, Old Road, Headington, Oxford OX3 7LD, UK.
Bone Joint Res. 2012 Mar 1;1(3):36-40. doi: 10.1302/2046-3758.13.2000032. Print 2012 Mar.
Surgical marking during tendon surgery is often used for technical and teaching purposes. This study investigates the effect of a gentian violet ink marker pen, a common surgical marker, on the viability of the tissue and cells of tendon.
In vitro cell and tissue methods were used to test the viability of human hamstring explants and the migrating tenocytes in the presence of the gentian violet ink.
The outcome of this study was that a constituent of the surgical marker pen causes cell and tissue death in culture, implying the same would occur in vivo.
This is a cause for concern when marking tendon during surgical procedures, as it may compromise healing and repair and potentially contribute to a poor outcome. The authors suggest that an alternative surgical marking procedure should be found, or that all marker pens should undergo testing on human tendon tissue in vitro prior to use.
在肌腱手术中,手术标记通常用于技术和教学目的。本研究调查了龙胆紫墨笔这种常见的手术标记物对肌腱组织和细胞活力的影响。
采用体外细胞和组织方法检测龙胆紫墨对人半腱肌外植体和迁移的肌腱细胞的活力。
本研究的结果表明,手术标记笔的一种成分在培养中导致细胞和组织死亡,这意味着在体内也会发生同样的情况。
当在手术过程中标记肌腱时,这是一个令人担忧的问题,因为它可能会影响愈合和修复,并可能导致不良结果。作者建议寻找替代的手术标记程序,或者在使用前对所有标记笔进行体外人肌腱组织测试。