Department of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, NE 68588, United States.
Biosens Bioelectron. 2013 Sep 15;47:408-14. doi: 10.1016/j.bios.2013.02.046. Epub 2013 Mar 21.
An optical method of mapping local redox reaction over a monolith electrode using simple laser scanning is described. As the optical signal is linearly proportional to the maximum redox current that is measured concomitantly by voltammetry, the optical signal quantitatively maps the local redox current density distribution. The method is demonstrated on two types of reactions: (1) a reversible reaction where the redox moieties are ionic, and (2) an irreversible reaction on two different types of enzymes immobilized on the electrode where the reaction moieties are nonionic. To demonstrate the scanning capability, the local redox behavior on a "V-shaped" electrode is studied where the local length scale and, hence, the local current density, is nonuniform. The ability to measure the current density distribution by this method will pave the way for multianalyte analysis on a monolith electrode using a standard three-electrode configuration. The method is called Scanning Electrometer for Electrical Double-layer (SEED).
本文描述了一种使用简单激光扫描对整体式电极上局部氧化还原反应进行映射的光学方法。由于光信号与通过伏安法同时测量的最大氧化还原电流呈线性比例，因此光信号定量地绘制了局部氧化还原电流密度分布。该方法在两种类型的反应上得到了验证：（1）氧化还原部分为离子的可逆反应，以及（2）两种不同类型的酶在电极上固定化的不可逆反应，其中反应部分为非离子的。为了证明扫描能力，研究了“V 形”电极上的局部氧化还原行为，其中局部长度尺度，因此局部电流密度是不均匀的。该方法通过这种方法测量电流密度分布的能力将为使用标准三电极配置在整体式电极上进行多分析物分析铺平道路。该方法称为双电层扫描静电计（SEED）。
