Department of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln (UNL), 207 Othmer Hall, Lincoln, NE 68588-0643, USA.
Department of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln (UNL), 207 Othmer Hall, Lincoln, NE 68588-0643, USA.
Biosens Bioelectron. 2014 Jul 15;57:41-7. doi: 10.1016/j.bios.2014.01.043. Epub 2014 Feb 2.
Redox on an electrode is an interfacial phenomenon that modulates the charge in the electrical double layer (EDL). A novel instrument, the Scanning Electrometer for Electrical Double-layer (SEED) has been developed to measure multiple enzyme reactions on a monolith electrode due to immunospecific binding with a mixture of respective analytes. SEED quantitatively maps the local redox reaction by scanning a laser on the array of enzyme monolayer spots immobilized on the monolith electrode. SEED measures the change in local charge state of the EDL that abruptly changes due to the redox reaction. The measurement spot size defined by the size of the laser beam is ~10 µm. The SEED signal is linearly proportional to the local redox current density and analyte concentration. The specificity is close to 100%. The SEED readout is compatible with microfluidics platform where the signal degrades less than 2% due to the poly(dimethyl siloxane) (PDMS) body.
电极上的氧化还原反应是一种界面现象，可调节双电层（EDL）中的电荷。由于与各自分析物的混合物具有免疫特异性结合，因此开发了一种新型仪器，即扫描电双层仪（SEED），以测量整体式电极上的多种酶反应。SEED 通过在固定在整体式电极上的酶单层斑点阵列上扫描激光来定量绘制局部氧化还原反应图。SEED 测量由于氧化还原反应而突然变化的 EDL 的局部电荷状态的变化。由激光束的大小定义的测量光斑大小约为 10 µm。SEED 信号与局部氧化还原电流密度和分析物浓度呈线性比例关系。特异性接近 100％。SEED 读数与微流控平台兼容，由于聚二甲基硅氧烷（PDMS）主体，信号的降解小于 2％。
