Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology, Daejeon 305-600, Republic of Korea.
Bioresour Technol. 2013 Jun;138:1-7. doi: 10.1016/j.biortech.2013.03.033. Epub 2013 Mar 16.
To produce fermentable sugar with fewer microbial inhibitors via few processes, batch-type hydrothermal treatments of sunflower stalks were performed, followed by enzymatic hydrolysis of pretreatment slurries with Cellic CTec2 and Cellic HTec2 (9:1, v/v, 0.1 ml/g dry biomass, 8.1FPU). The yields of hemicellulosic sugars were maximized at the pretreatment condition of 180°C for 30 min, while the furfural and 5-hydroxymethyl-2-furaldehyde (HMF) concentrations remained low. The glucose yield, however, was only 67.0% of the theoretical glucose yield (TGY). Either the treatment of raw biomass prior to pretreatment or the post-treatment of pretreated residue prior to enzymatic hydrolysis increased the glucose yield as follows: washing the pretreated solid with solvents (90% TGY)>partial removal of liquid fraction from the pretreatment slurry (PS, 83%)>removal of hot-water extractives from the biomass prior to pretreatment (77%)>prewetting of the biomass (70%).
为了通过较少的步骤生产出含有较少微生物抑制剂的可发酵糖，对向日葵秸秆进行了分批式水热处理，然后用 Cellic CTec2 和 Cellic HTec2（9:1，v/v，0.1 ml/g 干生物质，8.1FPU）对预处理浆料进行酶水解。在 180°C 下预处理 30 分钟的条件下，半纤维素糖的产率达到最大值，而糠醛和 5-羟甲基糠醛（HMF）的浓度保持较低。然而，葡萄糖的得率仅为理论葡萄糖得率（TGY）的 67.0%。在预处理之前对原料生物质进行处理或在酶解之前对预处理残渣进行后处理都可以提高葡萄糖得率，具体如下：用溶剂洗涤预处理的固体（90%TGY）>从预处理浆料中部分去除液体部分（PS，83%）>在预处理之前从生物质中去除热水提取物（77%）>在预处理之前对生物质进行预湿（70%）。
