Wang Y J, Zeng Q G, Xu L N
Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ministry of Education, Ningbo, China.
Genet Mol Res. 2013 Apr 2;12(2):892-900. doi: 10.4238/2013.April.2.6.
The blood clam, Tegillarca granosa, is widely cultivated in China. We isolated 6 microsatellite loci from T. granosa and used them to investigate genetic diversity and population structure of 5 widely distributed populations of blood clam collected from eastern and southeastern China. The allele number per locus varied from 4 to 9, and the polymorphism information content value was 0.301 to 0.830. The mean observed and expected heterozygosities varied from 0.304 to 0.460 and 0.556 to 0.621, respectively; the population from Yueqing had the smallest observed heterozygosity. In the neighbor-joining tree, Shandong, Fenghua and Yueqing populations clustered together, and there was geographic divergence between Shandong and Guangxi populations. Some microsatellite loci that were isolated from these mainland China samples were not found in blood clams collected from Malaysia.
泥蚶（Tegillarca granosa）在中国广泛养殖。我们从泥蚶中分离出6个微卫星位点，并用它们来研究从中国东部和东南部采集的5个广泛分布的泥蚶种群的遗传多样性和种群结构。每个位点的等位基因数从4到9不等，多态性信息含量值为0.301至0.830。观察到的平均杂合度和预期杂合度分别从0.304到0.460和0.556到0.621不等；乐清种群的观察到的杂合度最小。在邻接树上，山东、奉化和乐清种群聚在一起，山东和广西种群之间存在地理分化。从中国大陆这些样本中分离出的一些微卫星位点在从马来西亚采集的泥蚶中未被发现。
