Dong Yinghui, Yao Hanhan, Zhou Xiaolong, Lin Zhihua
Zhejiang Key Laboratory of Aquatic Germplasm Resources, College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, People's Republic of China.
College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai, People's Republic of China.
Genes Genomics. 2018 Apr;40(4):373-379. doi: 10.1007/s13258-017-0636-2. Epub 2017 Nov 25.
To determine the potential for productive efficiency and genetic improvement in the blood clam Tegillarca granosa, four offspring populations (ZZ, ZK, KZ and KK) were produced from a diallel mating of two different geographical stocks (Z and K). The levels of genetic diversity and population structures of four populations were analyzed using 14 polymorphic microsatellites. The results showed that the mean observed heterozygosities (Ho) of reciprocal cross populations (ZK and KZ) was higher than those of pure populations(ZZ and KK). The largest values of genetic differentiation coefficient (F = 0.067) and Nei's unbiased genetic distance (Dc = 0.263) were between ZK and KZ, and the smallest (F = 0.020, Dc = 0.116) were between ZZ and KK, which revealed that the largest genetic divergence was between the two reciprocal cross populations and the smallest was between two pure populations. This study demonstrated that the reciprocal cross populations of T. granosa had an extensive genetic difference and improvement, which may be advantageous for future breeding studies.
为了确定泥蚶（Tegillarca granosa）生产效率和遗传改良的潜力，通过两个不同地理种群（Z和K）的双列杂交产生了四个后代种群（ZZ、ZK、KZ和KK）。利用14个多态微卫星分析了四个种群的遗传多样性水平和种群结构。结果表明，正反交种群（ZK和KZ）的平均观察杂合度（Ho）高于纯种群（ZZ和KK）。遗传分化系数（F = 0.067）和Nei氏无偏遗传距离（Dc = 0.263）的最大值出现在ZK和KZ之间，最小值（F = 0.020，Dc = 0.116）出现在ZZ和KK之间，这表明两个正反交种群之间的遗传差异最大，而两个纯种群之间的遗传差异最小。本研究表明，泥蚶的正反交种群具有广泛的遗传差异和改良潜力，这可能对未来的育种研究有利。
