Department of Medical Oncology, Second Affiliated Hospital, Zhejiang University College of Medicine, Hangzhou 310000, Zhejiang Province, China.
World J Gastroenterol. 2013 Apr 21;19(15):2331-9. doi: 10.3748/wjg.v19.i15.2331.
To evaluate the expression of special AT-rich sequence-binding protein 1 (SATB1) gene in colorectal cancer and its role in colorectal cancer cell proliferation and invasion.
Immunohistochemistry was used to detect the protein expression of SATB1 in 30 colorectal cancer (CRC) tissue samples and pair-matched adjacent non-tumor samples. Cell growth was investigated after enhancing expression of SATB1. Wound-healing assay and Transwell assay were used to investigate the impact of SATB1 on migratory and invasive abilities of SW480 cells in vitro. Nude mice that received subcutaneous implantation or lateral tail vein were used to study the effects of SATB1 on tumor growth or metastasis in vivo.
SATB1 was over-expressed in CRC tissues and CRC cell lines. SATB1 promotes cell proliferation and cell cycle progression in CRC SW480 cells. SATB1 overexpression could promote cell growth in vivo. In addition, SATB1 could significantly raise the ability of cell migration and invasion in vitro and promote the ability of tumor metastasis in vivo. SATB1 could up-regulate matrix metalloproteases 2, 9, cyclin D1 and vimentin, meanwhile SATB1 could down-regulate E-cadherin in CRC.
SATB1 acts as a potential growth and metastasis promoter in CRC. SATB1 may be useful as a therapeutic target for CRC.
评估富含特殊 AT 序列结合蛋白 1（SATB1）基因在结直肠癌中的表达及其在结直肠癌细胞增殖和侵袭中的作用。
采用免疫组织化学方法检测 30 例结直肠癌（CRC）组织标本及其配对的相邻非肿瘤组织标本中 SATB1 的蛋白表达。增强 SATB1 的表达后，研究细胞生长情况。划痕愈合实验和 Transwell 实验用于研究 SATB1 对体外 SW480 细胞迁移和侵袭能力的影响。通过皮下植入或尾静脉注射裸鼠，研究 SATB1 对体内肿瘤生长或转移的影响。
SATB1 在 CRC 组织和 CRC 细胞系中过度表达。SATB1 促进 CRC SW480 细胞的增殖和细胞周期进程。SATB1 过表达可促进体内细胞生长。此外，SATB1 可显著提高细胞在体外的迁移和侵袭能力，并促进体内肿瘤转移能力。SATB1 可上调基质金属蛋白酶 2、9、细胞周期蛋白 D1 和波形蛋白，同时下调 CRC 中的 E-钙黏蛋白。
SATB1 作为 CRC 潜在的生长和转移促进因子。SATB1 可能是 CRC 治疗的一个有价值的靶点。
