Huang Bo, Xiong Fei, Wang Siwang, Lang Xianping, Wang Xiaodong, Zhou Hongli
Department of Thoracic Surgery, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121000, P.R. China.
Department of Nephropathy, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121000, P.R. China.
Oncol Lett. 2017 May;13(5):2915-2920. doi: 10.3892/ol.2017.5854. Epub 2017 Mar 13.
The aim of the present study was to investigate the effect of special AT-rich sequence-binding protein-1 (SATB1)-targeted small interfering RNA (siRNA) on the proliferation, invasion and apoptosis of TE-1 human esophageal cancer cells. SATB1 has been correlated with the metastasis and poor prognosis of colon and breast cancer, but the role of SATB1 in esophageal cancer remains unknown. Therefore, the present study constructed and transfected SATB1-siRNA into TE-1 cells in order to knockdown the expression of the SATB1 gene. Western blot analysis, a cell counting kit, transwell chamber assays and flow cytometry were used to assess the effect of SATB1-siRNA on the proliferation, invasion and apoptosis of cells. The results demonstrated that the expression of the SATB1 gene was efficiently knocked down by SATB1-siRNA, and that SATB1-siRNA inhibited the proliferation, invasion and apoptosis of TE-1 cells. Therefore, it was concluded that the SATB1 gene is important in the pathogenesis of human esophageal cancer, and may present a novel therapeutic target for esophageal cancer.
本研究的目的是探讨靶向富含特殊AT序列结合蛋白1（SATB1）的小干扰RNA（siRNA）对TE-1人食管癌细胞增殖、侵袭和凋亡的影响。SATB1与结肠癌和乳腺癌的转移及不良预后相关，但SATB1在食管癌中的作用尚不清楚。因此，本研究构建了SATB1-siRNA并将其转染至TE-1细胞中，以敲低SATB1基因的表达。采用蛋白质免疫印迹分析、细胞计数试剂盒、Transwell小室实验和流式细胞术评估SATB1-siRNA对细胞增殖、侵袭和凋亡的影响。结果表明，SATB1-siRNA有效敲低了SATB1基因的表达，且SATB1-siRNA抑制了TE-1细胞的增殖、侵袭和凋亡。因此，得出结论：SATB1基因在人食管癌发病机制中起重要作用，可能是食管癌的一个新的治疗靶点。
