Department of Circulation and Medical Imaging, Faculty of Medicine, The Norwegian University of Science and Technology, Trondheim, Norway.
PLoS One. 2013 Apr 17;8(4):e61578. doi: 10.1371/journal.pone.0061578. Print 2013.
In this study, the feasibility of high resolution magic angle spinning (HR MAS) magnetic resonance spectroscopy (MRS) of small tissue biopsies to distinguish between tumor and non-involved adjacent tissue was investigated. With the current methods, delineation of the tumor borders during breast cancer surgery is a challenging task for the surgeon, and a significant number of re-surgeries occur. We analyzed 328 tissue samples from 228 breast cancer patients using HR MAS MRS. Partial least squares discriminant analysis (PLS-DA) was applied to discriminate between tumor and non-involved adjacent tissue. Using proper double cross validation, high sensitivity and specificity of 91% and 93%, respectively was achieved. Analysis of the loading profiles from both principal component analysis (PCA) and PLS-DA showed the choline-containing metabolites as main biomarkers for tumor content, with phosphocholine being especially high in tumor tissue. Other indicative metabolites include glycine, taurine and glucose. We conclude that metabolic profiling by HR MAS MRS may be a potential method for on-line analysis of resection margins during breast cancer surgery to reduce the number of re-surgeries and risk of local recurrence.
在这项研究中，我们探讨了利用高分辨率魔角旋转（HR MAS）磁共振波谱（MRS）对小组织活检进行分析，以区分肿瘤组织和非受累相邻组织的可行性。在当前的方法中，乳腺癌手术中肿瘤边界的描绘对外科医生来说是一项具有挑战性的任务，并且有相当数量的手术需要再次进行。我们使用 HR MAS MRS 对 228 名乳腺癌患者的 328 个组织样本进行了分析。应用偏最小二乘判别分析（PLS-DA）来区分肿瘤组织和非受累相邻组织。通过适当的双交叉验证，获得了 91%的高灵敏度和 93%的高特异性。主成分分析（PCA）和 PLS-DA 的载荷分布分析显示，含有胆碱的代谢物是肿瘤含量的主要生物标志物，其中磷酰胆碱在肿瘤组织中含量特别高。其他有指示意义的代谢物包括甘氨酸、牛磺酸和葡萄糖。我们的结论是，HR MAS MRS 的代谢组学分析可能是一种潜在的方法，可以在乳腺癌手术中在线分析切缘，以减少再次手术的数量和局部复发的风险。
