Marczewska Jadwiga, Drozd Ewa, Anuszewska Elzbieta, Chilmonczyk Zdzisław, Łozowicka Bozena
National Medicines Institute, Biochemistry and Biopharmaceuticals Department, Chełmska 30/34, 00-725 Warszawa, Poland.
Acta Pol Pharm. 2013 Mar-Apr;70(2):349-54.
In our previous paper we examined mutagenic and genotoxic activity of 4 alpha-asarone isomers 2-5 exhibiting relatively high hypolipidemic activity. In the present paper, we examined genotoxic activity of alpha-asarone and its isomers as the ability to damage cellular DNA, evaluated in the comet assay. Additionally, mutagenic activity of alpha-asarone in Ames test has been examined. The Ames test for alpha-asarone was carried out in accordance with the guidelines of the PN-EN ISO 10993-3 standard. Compounds 4 and 5 were found to be devoid of any genotoxic activity while maintaining their hypolipemic potential. Because mutagenic activity of compound 4 was also minor it could be considered as a candidate for further pharmacological evaluation. Genotoxic but not mutagenic activity of alpha-asarone has been confirmed.
在我们之前的论文中，我们研究了表现出相对较高降血脂活性的4种α-细辛醚异构体2 - 5的致突变和遗传毒性活性。在本文中，我们通过彗星试验评估了α-细辛醚及其异构体作为损伤细胞DNA的能力的遗传毒性活性。此外，还研究了α-细辛醚在艾姆斯试验中的致突变活性。α-细辛醚的艾姆斯试验按照PN-EN ISO 10993-3标准的指南进行。发现化合物4和5在保持其降血脂潜力的同时没有任何遗传毒性活性。由于化合物4的致突变活性也较小，它可以被视为进一步药理学评估的候选物。已证实α-细辛醚具有遗传毒性但无致突变活性。
