Department of Microbiology and Molecular Medicine, CMU, Faculty of Medicine, University of Geneva, Rue Michel-Servet 1, CH-1211 Geneva 4, Switzerland.
Mol Microbiol. 2013 May;88(4):650-4. doi: 10.1111/mmi.12232. Epub 2013 Apr 24.
Possessing a system that experimentally controls gene expression has been a Holy Grail in molecular malaria research. Several strategies to control gene expression at different levels have been developed; the controlled step can range from transcription initiation to post-translational modification and/or protein degradation. Strategies successfully developed in model organisms and adapted to the malaria parasite can be classified into four categories aimed at the conditional control of (i) gene deletion, (ii) gene transcription, (iii) mRNA translation, and (iv) protein stability. Here, I intend to describe the various strategies available and compare and contrast their advantages and limitations. In the absence of a unique, ubiquitous solution, it is instrumental to utilize a variety of approaches that can respond to the particular needs of each gene.
在分子疟疾研究中，拥有一个能够对基因表达进行实验控制的系统一直是一个梦寐以求的目标。已经开发出了几种在不同水平上控制基因表达的策略；受控制的步骤可以从转录起始到翻译后修饰和/或蛋白质降解。在模式生物中成功开发并适用于疟原虫的策略可以分为四类，旨在对（i）基因缺失、（ii）基因转录、（iii）mRNA 翻译和（iv）蛋白质稳定性进行条件控制。在这里，我打算描述可用的各种策略，并比较和对比它们的优缺点。在缺乏通用解决方案的情况下，使用能够响应每个基因特定需求的多种方法是非常重要的。
