植物中茉莉酸应答凝集素的表达分析

Expression analysis of jasmonate-responsive lectins in plants.

作者信息

Lannoo Nausicaä, Van Damme Els J M

机构信息

Laboratory of Glycobiology and Biochemistry, Department of Molecular Biotechnology, Faculty of Bioscience Engineering, Ghent University, Gent, Belgium.

出版信息

Methods Mol Biol. 2013;1011:251-63. doi: 10.1007/978-1-62703-414-2_20.

DOI:10.1007/978-1-62703-414-2_20

PMID:23616002

Abstract

The Nicotiana tabacum lectin, also designated Nictaba, is a nucleocytoplasmic carbohydrate-binding protein produced in tobacco leaves after application of specific jasmonates and upon insect herbivory. Here, we describe different techniques by which lectin production can be induced through exogenous jasmonate application on tobacco plants. Furthermore, we elaborate on the assays to detect Nictaba expression at RNA and protein levels as well as on the agglutination assays to identify the lectin activity.

摘要

烟草凝集素，也被称为Nictaba，是一种核质碳水化合物结合蛋白，在施加特定茉莉酸酯后以及受到昆虫取食时在烟草叶片中产生。在此，我们描述了通过在烟草植株上外源施加茉莉酸酯来诱导凝集素产生的不同技术。此外，我们详细阐述了在RNA和蛋白质水平检测Nictaba表达的分析方法以及用于鉴定凝集素活性的凝集分析方法。