Lannoo Nausicaä, Vandenborre Gianni, Miersch Otto, Smagghe Guy, Wasternack Claus, Peumans Willy J, Van Damme Els J M
Laboratory of Biochemistry and Glycobiology, Department of Molecular Biotechnology, Ghent University, Coupure Links 653, B-9000 Gent, Belgium.
Plant Cell Physiol. 2007 Aug;48(8):1207-18. doi: 10.1093/pcp/pcm090. Epub 2007 Jul 10.
Previous experiments with tobacco (Nicotiana tabacum L. cv Samsun NN) plants revealed that jasmonic acid methyl ester (JAME) induces the expression of a cytoplasmic/nuclear lectin in leaf cells and provided the first evidence that jasmonates affect the expression of carbohydrate-binding proteins in plant cells. To corroborate the induced accumulation of relatively large amounts of a cytoplasmic/nuclear lectin, a detailed study was performed on the induction of the lectin in both intact tobacco plants and excised leaves. Experiments with different stress factors demonstrated that the lectin is exclusively induced by exogeneously applied jasmonic acid and JAME, and to a lesser extent by insect herbivory. The lectin concentration depends on leaf age and the position of the tissue in the leaf. JAME acts systemically in intact plants but very locally in excised leaves. Kinetic analyses indicated that the lectin is synthesized within 12 h exposure time to JAME, reaching a maximum after 60 h. After removal of JAME, the lectin progressively disappears from the leaf tissue. The JAME-induced accumulation of an abundant nuclear/cytoplasmic lectin is discussed in view of the possible role of this lectin in the plant.
先前对烟草（Nicotiana tabacum L. cv Samsun NN）植株进行的实验表明，茉莉酸甲酯（JAME）可诱导叶细胞中一种胞质/核凝集素的表达，并首次证明茉莉酸酯会影响植物细胞中碳水化合物结合蛋白的表达。为了证实相对大量的胞质/核凝集素的诱导积累，我们对完整烟草植株和离体叶片中凝集素的诱导情况进行了详细研究。不同胁迫因子的实验表明，凝集素仅由外源施加的茉莉酸和茉莉酸甲酯诱导产生，在较小程度上也可由昆虫取食诱导产生。凝集素浓度取决于叶片年龄和叶片中组织的位置。茉莉酸甲酯在完整植株中具有系统性作用，但在离体叶片中作用非常局部。动力学分析表明，凝集素在暴露于茉莉酸甲酯12小时内合成，60小时后达到最大值。去除茉莉酸甲酯后，凝集素逐渐从叶片组织中消失。鉴于这种凝集素在植物中的可能作用，我们讨论了茉莉酸甲酯诱导的丰富核/胞质凝集素的积累情况。
