Department of Renal and Urologic Surgery, Asahikawa Medical University, 2-1-1-1, Midorigaoka-Higashi, Asahikawa, Hokkaido, 078-8510, Japan.
Int Urol Nephrol. 2013 Jun;45(3):749-54. doi: 10.1007/s11255-013-0442-y. Epub 2013 Apr 25.
To investigate bladder function in a model of nonbacterial prostatitis (NBP) induced in castrated rats by 17β-estradiol injection.
Ten-month-old male Wistar rats were divided into two groups, sham and NBP (both N = 8). NBP was induced by castration followed by daily subcutaneous injection of 17β-estradiol for 30 days. On the 31st day after surgery, we investigated (1) voiding behavior, (2) bladder blood flow (BBF), (3) prostate and bladder weight, and proinflammatory cytokines (TNF-α and CXCL1) levels and (4) bladder contractile responses to electrical field stimulation (EFS), carbachol and KCl.
(1) Voiding behavior (average micturition volume, total urine volume and number of micturitions) and (2) BBF were not significantly different between the sham and NBP groups. (3) NBP led to a significant decrease in prostatic weight and increase in proinflammatory cytokine levels in the prostate, but NBP did not cause a significant change in bladder weight or proinflammatory cytokine levels in the bladder. (4) Bladder contractile forces in response to EFS, carbachol and KCl were not significantly affected by NBP.
In this rat model, NBP did not cause a significant change in the level of proinflammatory cytokines in the bladder and affect bladder function.
研究 17β-雌二醇注射诱导去势大鼠非细菌性前列腺炎（NBP）模型中的膀胱功能。
将 10 月龄雄性 Wistar 大鼠分为假手术组和 NBP 组（每组 N = 8）。NBP 通过去势后每天皮下注射 17β-雌二醇 30 天诱导。术后第 31 天，我们研究了（1）排尿行为，（2）膀胱血流（BBF），（3）前列腺和膀胱重量，以及促炎细胞因子（TNF-α 和 CXCL1）水平，（4）膀胱对电刺激（EFS）、卡巴胆碱和 KCl 的收缩反应。
（1）排尿行为（平均排尿量、总尿量和排尿次数）和（2）BBF 在假手术组和 NBP 组之间没有显著差异。（3）NBP 导致前列腺重量显著降低，前列腺中促炎细胞因子水平升高，但 NBP 并未导致膀胱重量或膀胱中促炎细胞因子水平发生显著变化。（4）EFS、卡巴胆碱和 KCl 引起的膀胱收缩力不受 NBP 的影响。
在该大鼠模型中，NBP 不会引起膀胱中促炎细胞因子水平的显著变化，也不会影响膀胱功能。
