Department of Pesticide Science, College of Plant Protection, Nanjing Agricultural University, Jiangsu Key Laboratory of Pesticide Science, Ministry of Education, Nanjing 210095, PR China.
Analyst. 2013 Jun 7;138(11):3280-6. doi: 10.1039/c3an00205e.
A novel and sensitive enhanced chemiluminescence enzyme-linked immunosorbent assay (ECL-ELISA) for the simultaneous analysis of imidaclothiz and thiacloprid is described. The concentrations of coating antibodies and enzyme tracers were optimised by three different ECL-ELISA formats. Under the optimised conditions, when anti-imidaclothiz polyclonal and anti-thiacloprid polyclonal antibodies were immobilised in a single well, single-enzyme tracer ECL-ELISA was used to screen the corresponding pesticide residue, and multi-enzyme tracer ECL-ELISA was used for the analysis of imidaclothiz and thiacloprid residue mixtures. The average recoveries of the two pesticides from spiked tomato, cabbage, and rice samples were in the range of 83.7-117%. Meanwhile, the results showed that the multi-enzyme tracer ECL-ELISA could be applied to the accurate analysis of different proportions of imidaclothiz and thiacloprid (1 : 4 to 4 : 1, m/m) when the gross residues ranged from 20 to 250 μg kg(-1). The results of the multi-enzyme tracer ECL-ELISA in the real tomato samples correlated well with those of high-performance liquid chromatography, with a correlation coefficient of 0.996. Therefore, this new strategy for developing immunoassays is suitable for the simultaneous quantitative detection of imidaclothiz and thiacloprid residues in agricultural samples.
一种新颖且灵敏的增强化学发光酶联免疫吸附测定法（ECL-ELISA），用于同时分析吡虫脒和噻虫啉。通过三种不同的 ECL-ELISA 格式优化了包被抗体和酶示踪剂的浓度。在优化条件下，当抗吡虫脒多克隆抗体和抗噻虫啉多克隆抗体固定在单个孔中时，使用单酶示踪剂 ECL-ELISA 筛选相应的农药残留，并用多酶示踪剂 ECL-ELISA 分析吡虫脒和噻虫啉残留混合物。两种农药从添加的番茄、白菜和大米样品中的平均回收率在 83.7-117%范围内。同时，结果表明，当总残留量在 20-250μgkg(-1)范围内时，多酶示踪剂 ECL-ELISA 可用于准确分析吡虫脒和噻虫啉不同比例（1:4 至 4:1，m/m）的混合物。在实际番茄样品中，多酶示踪剂 ECL-ELISA 的结果与高效液相色谱法的结果相关性良好，相关系数为 0.996。因此，这种开发免疫分析的新策略适用于同时定量检测农业样品中的吡虫脒和噻虫啉残留。
