School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
Analyst. 2013 Jun 7;138(11):3259-63. doi: 10.1039/c3an00206c.
This work creatively uses peroxidase-like ZnS hollow spheres (ZnS HSs) to cooperate with glucose oxidase (GOx) for glucose determinations. This approach is that the ZnS HSs electrocatalytically oxidate the enzymatically generated H2O2 to O2, and then the O2 circularly participates in the previous glucose oxidation by glucose oxidase. Au nanoparticles (AuNPs) and carbon nanotubes (CNTs) are used as electron transfer and enzyme immobilization matrices, respectively. The biosensor of glucose oxidase-carbon nanotubes-Au nanoparticles-ZnS hollow spheres-gold electrode (GOx-CNT-AuNPs-ZnS HSs-GE) exhibits a rapid response, a low detection limit (10 μM), a wide linear range (20 μM to 7 mM) as well as good anti-interference, long-term longevity and reproducibility.
这项工作创造性地使用过氧化物酶样的 ZnS 空心球（ZnS HSs）与葡萄糖氧化酶（GOx）协同作用来测定葡萄糖。这种方法是通过 ZnS HSs 电催化酶促产生的 H2O2 氧化为 O2，然后 O2 通过葡萄糖氧化酶循环参与之前的葡萄糖氧化。金纳米粒子（AuNPs）和碳纳米管（CNTs）分别用作电子转移和酶固定基质。葡萄糖氧化酶-碳纳米管-金纳米粒子-ZnS 空心球-金电极的生物传感器（GOx-CNT-AuNPs-ZnS HSs-GE）表现出快速响应、低检测限（10 μM）、宽线性范围（20 μM 至 7 mM）以及良好的抗干扰性、长期稳定性和可重复性。
