Franklin C, Goldstein L
Enzyme. 1975;19(4):212-24. doi: 10.1159/000458994.
Properties of renal phosphate-dependent glutaminase (EC 3.5.1.2), assayed in tissue homogenates, were compared in adult, 2-week-old, and newborn rats. Vmax, Km glutamine, pH optimum, inhibition by glutamate, activation by phosphate, intracellular distribution, and the possible presence of activators or inhibitors were examined. Although Vmax increased threefold during postnatal development, no major differences in the properties of the enzyme at the three stages of development were noted. It was concluded that the enzyme protein remains the same throughout development, both in biochemical properties and intracellular location, but that more of it is accumulated or is converted to an active state as the kidney matures.
在成年大鼠、2周龄大鼠和新生大鼠中，对在组织匀浆中测定的肾磷酸依赖性谷氨酰胺酶（EC 3.5.1.2）的特性进行了比较。研究了最大反应速度（Vmax）、谷氨酰胺的米氏常数（Km）、最适pH值、谷氨酸的抑制作用、磷酸盐的激活作用、细胞内分布以及可能存在的激活剂或抑制剂。尽管在出生后的发育过程中Vmax增加了三倍，但在发育的三个阶段，该酶的特性没有明显差异。得出的结论是，在整个发育过程中，酶蛋白在生化特性和细胞内定位方面保持不变，但随着肾脏成熟，更多的酶蛋白被积累或转化为活性状态。
