Das Bodhisatwa, Fernandez Francis, John Annie, Sharma Chandra P
Biosurface Technology Division, SCTIMST, Trivandrum, India.
J Stem Cells. 2012;7(3):189-99.
Quantum clusters are sub-nano sized materials mostly synthesized from noble metals with luminescence property and high quantum yield. They are important to biomedical scientists because of their excellent optical properties. Here we represent a tool for cell imaging purpose using protein stabilized gold quantum clusters. Intestinal protease Trypsin was used to develop clusters. They were conjugated to cyclic RGD peptides by EDAC coupling. Cell imaging property was checked by transfecting the RGD-conjugated quantum clusters to bone marrow stem cells. For characterization of RGD-conjugated quantum clusters UV-Vis, Fluorescence and FTIR spectroscopy was performed. DLS and Zeta potential measurement also have been done. To check the bio compatibility of the quantum clusters MTT assay, AFM and blood cell adhesion study were performed. The samples are found out to be good for cell imaging as well as bio compatible and hemo-compatible.
量子簇是亚纳米尺寸的材料，主要由具有发光特性和高量子产率的贵金属合成。由于其优异的光学性质，它们对生物医学科学家来说很重要。在这里，我们展示了一种使用蛋白质稳定的金量子簇用于细胞成像的工具。肠道蛋白酶胰蛋白酶被用于制备量子簇。它们通过EDAC偶联与环状RGD肽缀合。通过将RGD缀合的量子簇转染到骨髓干细胞中来检查细胞成像特性。对RGD缀合的量子簇进行了紫外可见光谱、荧光光谱和傅里叶变换红外光谱表征。还进行了动态光散射和zeta电位测量。为了检查量子簇的生物相容性，进行了MTT测定、原子力显微镜和血细胞粘附研究。发现这些样品对细胞成像以及生物相容性和血液相容性都很好。
