通过阴离子交换色谱法和安培检测法测定胍基化合物。

Determination of guanidino compounds by anion-exchange chromatography and amperometric detection.

作者信息

Ding W D, Veening H, Van Effen R M

机构信息

Department of Chemistry, Bucknell University, Lewisburg, PA 17837.

出版信息

J Chromatogr. 1990 Apr 6;526(2):355-66. doi: 10.1016/s0378-4347(00)82519-0.

DOI:10.1016/s0378-4347(00)82519-0

PMID:2361978

Abstract

Guanidino compounds were separated and determined by anion-exchange chromatography and electrochemical detection using a basic aqueous eluent and a nickel working electrode. It was found necessary to use a sample clean-up procedure prior to chromatographic analysis of uremic dialysate and serum samples. The effect of eluent hydroxide concentration on the retention of guanidino compounds was studied. Quantitative calibration showed that working curves were non-linear. Electrochemical detection for guanidino compounds with a nickel working electrode, while not selective, has high detection sensitivity. Detection limits for guanidino compounds ranged from 3 to 12 pmol.

摘要

采用碱性水性洗脱液和镍工作电极，通过阴离子交换色谱法和电化学检测法对胍基化合物进行分离和测定。发现对尿毒症透析液和血清样品进行色谱分析之前，有必要采用样品净化程序。研究了洗脱液氢氧化物浓度对胍基化合物保留的影响。定量校准表明工作曲线是非线性的。用镍工作电极对胍基化合物进行电化学检测，虽然不具有选择性，但具有很高的检测灵敏度。胍基化合物的检测限为3至12皮摩尔。