De Deyn P, Marescau B, Lornoy W, Becaus I, Van Leuven I, Van Gorp L, Lowenthal A
Nephron. 1987;45(4):291-5. doi: 10.1159/000184166.
Guanidino compounds are increased in uremia and are highly suspected to be uremic toxins. The serum levels of 11 guanidino compounds and the influence of a single hemodialysis were evaluated in 30 steady-state uremic patients undergoing maintenance hemodialysis. Guanidino compound levels were detected using liquid cation exchange chromatography with a highly sensitive fluorescence detection method. Highly standardized dialysis procedures were performed. Before hemodialysis, high levels were found for guanidinosuccinic acid, N-alpha-acetylarginine, argininic acid, creatinine, gamma-guanidinobutyric acid, guanidine and methylguanidine. Guanidinosuccinic acid reached levels associated with toxic effects in vitro. After hemodialysis, although lowered, guanidinosuccinic acid, creatinine, guanidine and methylguanidine were still markedly increased. No differences in the percent decrease, during a single hemodialysis, of the studied compounds were found using different membranes such as cellulose acetate, cuprophane and polyacrylonitrile membranes. Substantial differences, however, in the percent decrease of the different guanidino compounds were found, ranging from 25 +/- 13% for arginine to 74 +/- 7.5% for guanidinosuccinic acid. Data reported here show that guanidino compounds are raised in serum of uremic patients undergoing maintenance hemodialysis, before as well as after a single hemodialysis, while substantial differences in the percent decrease of the different guanidino compounds are found.
胍基化合物在尿毒症患者中升高，高度怀疑是尿毒症毒素。对30例接受维持性血液透析的稳定期尿毒症患者评估了11种胍基化合物的血清水平及单次血液透析的影响。采用具有高灵敏度荧光检测方法的液相阳离子交换色谱法检测胍基化合物水平。实施了高度标准化的透析程序。血液透析前，胍基琥珀酸、N-α-乙酰精氨酸、精氨酸酸、肌酐、γ-胍基丁酸、胍和甲基胍水平较高。胍基琥珀酸达到了体外与毒性作用相关的水平。血液透析后，尽管胍基琥珀酸、肌酐、胍和甲基胍有所降低，但仍显著升高。使用不同膜（如醋酸纤维素膜、铜氨膜和聚丙烯腈膜）进行单次血液透析时，所研究化合物的降低百分比没有差异。然而，不同胍基化合物的降低百分比存在显著差异，从精氨酸的25±13%到胍基琥珀酸的74±7.5%不等。此处报告的数据表明，接受维持性血液透析的尿毒症患者血清中的胍基化合物在单次血液透析前后均升高，同时不同胍基化合物的降低百分比存在显著差异。
