通过高效液相色谱法对洋地黄毒苷的3,5-二硝基苯甲酰衍生物进行电化学检测。

Electrochemical detection of the 3,5-dinitrobenzoyl derivative of digoxin by high-performance liquid chromatography.

作者信息

Embree L, McErlane K M

机构信息

Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, Canada.

出版信息

J Chromatogr. 1990 Apr 6;526(2):439-46. doi: 10.1016/s0378-4347(00)82526-8.

DOI:10.1016/s0378-4347(00)82526-8

PMID:2361983

Abstract

Electrochemical detection of 3,5-dinitrobenzoyl derivatives of digoxin and its metabolites following high-performance liquid chromatography is reported. Partial resolution of derivatized digoxin and dihydrodigoxin was obtained using a Spherisorb ODS II analytical column. Both single- and dual-electrode detection were investigated and a maximum sensitivity equivalent to 0.39 ng of digoxin was found with the dual-electrode method. This system has the necessary sensitivity and selectivity for development into a therapeutic monitoring assay method.

摘要

报道了高效液相色谱法之后对地高辛及其代谢物的3,5-二硝基苯甲酰衍生物进行电化学检测的情况。使用Spherisorb ODS II分析柱实现了衍生化地高辛和二氢地高辛的部分分离。对单电极和双电极检测都进行了研究，双电极法的最大灵敏度相当于0.39纳克地高辛。该系统具有开发成治疗监测测定方法所需的灵敏度和选择性。