Plum J, Daldrup T
J Chromatogr. 1986 Apr 25;377:221-31. doi: 10.1016/s0378-4347(00)80777-x.
High-performance liquid chromatographic (HPLC) systems are described for the separation of the cardioactive metabolites of the digoxin and digitoxin series that are formed by the splitting of digitoxose sugar residues from the aglycone steroid: isocratic separation of digoxin and its cardioactive metabolites; isocratic separation of digitoxin and its cardioactive metabolites; and gradient elution separation of the digoxin and digitoxin series including beta-acetyl- and beta-methyldigoxin. Separations were performed on a 10-microns bonded octadecyl phase column using various mixtures of acetonitrile--water and acetonitrile--methanol--water as the mobile phase. These methods provide high peak resolution and are well suited for collecting elution fractions, e.g. to link up with sensitive immunological measurements. An HPLC-radioimmunoassay method is described for the quantitation of digoxin, digitoxin and their metabolites in human tissues.
描述了高效液相色谱（HPLC）系统用于分离地高辛和洋地黄毒苷系列的心脏活性代谢产物，这些代谢产物是通过从苷元甾体上裂解洋地黄毒糖残基形成的：地高辛及其心脏活性代谢产物的等度分离；洋地黄毒苷及其心脏活性代谢产物的等度分离；以及包括β-乙酰基和β-甲基地高辛在内的地高辛和洋地黄毒苷系列的梯度洗脱分离。在10微米键合十八烷基相柱上进行分离，使用乙腈 - 水以及乙腈 - 甲醇 - 水的各种混合物作为流动相。这些方法提供了高的峰分辨率，非常适合收集洗脱馏分，例如与灵敏的免疫测定法联用。描述了一种HPLC - 放射免疫测定法用于定量人体组织中的地高辛、洋地黄毒苷及其代谢产物。
