Maloney J A, Awni W M
Drug Evaluation Unit, Hennepin County Medical Center, Minneapolis, MN 55415.
J Chromatogr. 1990 Apr 6;526(2):487-96. doi: 10.1016/s0378-4347(00)82530-x.
A high-performance liquid chromatographic method for the measurement of isepamicin, a new aminoglycoside, in plasma, urine and dialysate is reported. The assay utilizes a simple extraction of isepamicin in plasma using commercially available Cyano solid-phase cartridges and dilution of urine and dialysate samples. The separation is performed on a Hypersil C18 column (15 cm X 4.6 mm I.D., 5 microns particle size) and utilizes a mobile phase consisting of 10% methanol and 90% buffer solution containing 0.01 M sodium hexanesulfonate, 0.1 M sodium sulfate and 17 mM acetic acid. The flow-rate is 1.1 ml/min. Dibekacin is used as the internal standard. Isepamicin is derivatized post-column with o-phthalaldehyde for spectrofluorometric detection. The method can also be used for the measurement of other aminoglycosides, i.e. tobramycin, kanamycin, netilmicin and gentamicin. The assay is fast, accurate and has a quantitation limit of 100 ng/ml isepamicin in plasma and 50 ng/ml in urine and dialysate.
本文报道了一种高效液相色谱法，用于测定血浆、尿液和透析液中的新氨基糖苷类药物异帕米星。该测定方法采用市售氰基固相柱对血浆中的异帕米星进行简单萃取，并对尿液和透析液样品进行稀释。分离在Hypersil C18柱（15 cm×4.6 mm内径，5微米粒径）上进行，流动相由10%甲醇和90%缓冲溶液组成，缓冲溶液含有0.01 M己烷磺酸钠、0.1 M硫酸钠和17 mM乙酸。流速为1.1 ml/min。地贝卡星用作内标。异帕米星在柱后用邻苯二甲醛衍生化，用于荧光分光光度检测。该方法还可用于测定其他氨基糖苷类药物，即妥布霉素、卡那霉素、奈替米星和庆大霉素。该测定方法快速、准确，血浆中异帕米星的定量限为100 ng/ml，尿液和透析液中为50 ng/ml。
