Forest J M, Mailhot C, Cartier P, Sirois G
Hôpital Ste-Justine, Montréal, Québec, Canada.
Ther Drug Monit. 1997 Feb;19(1):74-8. doi: 10.1097/00007691-199702000-00013.
The quantitative analysis of netilmicin in plasma, peritoneal dialysate, and urine using the fluorescence polarization immunoassay (FPIA) of the Abbott TDx system is compared with the modified high-performance liquid chromatography (HPLC) method of Peng et al., which was chosen as a reference. Using the least square method, we found that the results of the FPIA (y) correlated well with those obtained with HPLC (x). The three regression equations for the plasma, peritoneal dialysate, and urine samples, respectively, were y = 0.71x + 0.44 with r = 0.88 and n = 45; y = 0.94x + 1.22 with r = 0.93 and n = 95; and y = 0.92x + 0.70 with r = 0.93 and n = 61. The corresponding mean errors (FPIA-HPLC) with their 95% confidence intervals were -0.19 (-0.38 to -0.02), 0.69 (-0.42 to 1.81), and -0.13 (-1.13 to 0.87) microgram/ml. According to results of the Wilcoxon matched-pairs signed-ranks test, these errors did not represent a significant bias. The FPIA is thus suitable for analyzing netilmicin in the three biological fluids studied except when dialysate is contaminated with Amuchina. In this case, HPLC should be used.
使用雅培TDx系统的荧光偏振免疫分析法（FPIA）对血浆、腹膜透析液和尿液中的奈替米星进行定量分析，并与彭等人改进的高效液相色谱法（HPLC）进行比较，后者被选作参考方法。通过最小二乘法，我们发现FPIA的结果（y）与HPLC的结果（x）具有良好的相关性。血浆、腹膜透析液和尿液样本的三个回归方程分别为：y = 0.71x + 0.44，r = 0.88，n = 45；y = 0.94x + 1.22，r = 0.93，n = 95；y = 0.92x + 0.70，r = 0.93，n = 61。相应的平均误差（FPIA - HPLC）及其95%置信区间分别为-0.19（-0.38至-0.02）、0.69（-0.42至1.81）和-0.13（-1.13至0.87）微克/毫升。根据Wilcoxon配对符号秩检验的结果，这些误差并不代表显著偏差。因此，FPIA适用于分析所研究的三种生物流体中的奈替米星，除非透析液被阿穆奇纳污染。在这种情况下，应使用HPLC。
