Department of Animal Science, University of Manitoba, Winnipeg, Manitoba R3T 2N2, Canada.
Cell Biol Int. 2013 Sep;37(9):961-71. doi: 10.1002/cbin.10122. Epub 2013 May 28.
We have examined the effect of oleic acid (OA) concentrations and incubation time, along with chicken serum (CS), on adipogenic differentiation and expression of adipogenic transcripts in hen preadipocytes. Preadipocytes were treated with (i) an adipogenic cocktail (DMI) containing 500 nM dexamethasone, 0.5 mM 3-isobutyl-1-methylxanthine and 20 µg/mL insulin alone and DMI + 75, 150, 300 or 600 µM OA for 48 h; (ii) DMI + 300 µM OA (DMIOA) for 6, 12, 24 or 48 h; and (iii) foetal bovine serum (FBS), CS, DMI + FBS, DMI + CS, DMIOA + FBS and DMIOA + CS. While FABP4 was significantly expressed with increasing concentrations of OA, the expression of C/EBPβ, LEPR and FAS were unchanged compared with the control. PPARγ2 expression was unchanged across all time-points. A significantly higher level of C/EBPα was measured at 48 h, but the levels of C/EBPβ increased after 12 h. Levels of FABP4 significantly increased with the time of incubation after 12 h, but that of LPL was reduced (P < 0.05) at 6, 24 and 48 h. FABP4 was highly expressed in cells treated with CS, DMI + CS and DMIOA + CS compared to cells treated with FBS, DMI + FBS and DMIOA + FBS. In conclusion, increased concentrations of OA and incubation time increases lipid accumulation; FABP4 and C/EBPβ are potential transcription factors regulating OA induced adipogenesis of fat cells obtained from laying hen. CS is a potent inducer of adipogenic differentiation in hen preadipocytes.
我们研究了油酸（OA）浓度和孵育时间以及鸡血清（CS）对母鸡前体脂肪细胞的成脂分化和成脂转录物表达的影响。前体脂肪细胞用（i）含有 500 nM 地塞米松、0.5 mM 3-异丁基-1-甲基黄嘌呤和 20 µg/mL 胰岛素的成脂鸡尾酒（DMI）单独处理，以及 DMI+75、150、300 或 600 µM OA 处理 48 h；（ii）DMI+300 µM OA（DMIOA）处理 6、12、24 或 48 h；和（iii）胎牛血清（FBS）、CS、DMI+FBS、DMI+CS、DMIOA+FBS 和 DMIOA+CS。虽然 FABP4 的表达随着 OA 浓度的增加而显著增加，但与对照相比，C/EBPβ、LEPR 和 FAS 的表达没有变化。PPARγ2 的表达在所有时间点都没有变化。在 48 h 时测量到 C/EBPα 的水平显著升高，但 C/EBPβ 的水平在 12 h 后增加。FABP4 的水平在孵育 12 h 后随着时间的推移显著增加，但 LPL 的水平在 6、24 和 48 h 时降低（P<0.05）。与用 FBS、DMI+FBS 和 DMIOA+FBS 处理的细胞相比，用 CS、DMI+CS 和 DMIOA+CS 处理的细胞中 FABP4 的表达高度增加。总之，OA 浓度的增加和孵育时间的延长会增加脂肪堆积；FABP4 和 C/EBPβ 可能是调节鸡脂肪细胞中 OA 诱导的成脂分化的潜在转录因子。CS 是诱导母鸡前体脂肪细胞成脂分化的有效诱导剂。
