Regassa Alemu, Park Kye Won, Kim Woo Kyun
Department of Animal Science, University of Manitoba, Winnipeg, Manitoba, Canada.
Department of Food Science and Biotechnology, Sungkyunkwan University, Suwon, 16419, Republic of Korea.
Cell Biol Int. 2016 Oct;40(10):1123-8. doi: 10.1002/cbin.10651. Epub 2016 Aug 9.
A study was conducted to examine the effect of phenamil on adipogenic differentiation and expression of key adipogenic transcripts in hen preadipocytes. Preadipocytes were isolated from 20-week old Single Comb White Leghorn hens (Gallas gallus, Lohman strain). The experiment lasted for 48 h and had six treatments. Non-treated control (C) cells, cells treated with dexamethasone, 3-isobutyl-1-methylxanthine, insulin, and oleic acid (DMIOA) (T1), DMIOA + 15 μM phenamil (T2), DMIOA + 30 μM phenamil (T3), 15 μM phenamil alone (T4), and 30 μM phenamil alone (T5). Neutral lipid accumulation and the mRNA expression of key adipogenic transcripts were measured in all treatments and compared. Lipid accumulation was detected in T1, T2, and T3 only. Expression of peroxisome proliferator receptor-activator gamma 2 (PPARγ2), the core enhancer binding protein α (C/EBPα), C/EBPβ, fatty acid binding protein 4 (FABP4), and lipoprotein lipase (LPL) as well as ETS variant 4 (ETV4) and 5 was higher (P < 0.05) in T2, T3, T4, and T5 compared to C. Expression of these transcripts was higher (P < 0.05) in T2 and T3 compared to T4 and T5. The core enhancer binding protein α, C/EBPβ, and FABP4 were highly expressed (P < 0.05) in T1 compared to C. However, the expression of PPARγ2, LPL, and ETV4 and ETV5 was not significantly different. Expression of C/EBPα, C/EBPβ, and FABP4 was higher (P < 0.05) in T2 and T3 compared to T1. Expression of sterol regulatory element binding protein 1 (SREBP1) and leptin receptor (LEPR) was not significantly different among the treatments. In conclusion, phenamil enhances DMIOA-induced adipogenic differentiation of hen preadipocytes but does not induce adipogenesis by itself.
进行了一项研究，以检验苯那米对母鸡前脂肪细胞成脂分化及关键成脂转录本表达的影响。从前脂肪细胞取自20周龄单冠白来航鸡（原鸡属，罗曼品系）。实验持续48小时，有六种处理方式。未处理的对照（C）细胞、用地塞米松、3-异丁基-1-甲基黄嘌呤、胰岛素和油酸处理的细胞（DMIOA）（T1）、DMIOA + 15 μM苯那米（T2）、DMIOA + 30 μM苯那米（T3）、单独使用15 μM苯那米（T4）和单独使用30 μM苯那米（T5）。在所有处理中测量中性脂质积累及关键成脂转录本的mRNA表达并进行比较。仅在T1、T2和T3中检测到脂质积累。与C相比，T2、T3、T4和T5中过氧化物酶体增殖物激活受体γ2（PPARγ2）、核心增强子结合蛋白α（C/EBPα）、C/EBPβ、脂肪酸结合蛋白4（FABP4）和脂蛋白脂肪酶（LPL）以及ETS变异体4（ETV4）和5的表达更高（P < 0.05）。与T4和T5相比，T2和T3中这些转录本的表达更高（P < 0.05）。与C相比，T1中核心增强子结合蛋白α、C/EBPβ和FABP4高表达（P < 0.05）。然而，PPARγ2、LPL以及ETV4和ETV5的表达无显著差异。与T1相比，T2和T3中C/EBPα、C/EBPβ和FABP4的表达更高（P < 0.05）。固醇调节元件结合蛋白1（SREBP1）和瘦素受体（LEPR）在各处理间表达无显著差异。总之，苯那米增强了DMIOA诱导的母鸡前脂肪细胞成脂分化，但自身并不诱导脂肪生成。
