Chinese Medicine Toxicological Laboratory, Institute of Traditional Chinese Medicine, Heilongjiang University of Chinese Medicine, Harbin, 150040, PR China.
Phytother Res. 2014 Feb;28(2):280-8. doi: 10.1002/ptr.4997. Epub 2013 Apr 26.
Rhizoma Dioscoreae Nipponicae (RDN) is a widely used traditional Chinese herb, which is used to treat arthroncus, arthrodynia and arthritis. As is known to us, inflammatory mechanisms have played an important role in the occurrence, course and prognosis of gouty arthritis (GA). The aim of this study was to determine the characteristic expressed proteins of synovium in GA rat and synovial cell. The rat model of GA was induced by monosodium urate (MSU) crystal. Tissue samples were assayed by immunohistochemical method. The effects of RDN on Stromal cell-derived factor 1 (SDF-1), CXCR 4 and p38 mitogen-activated protein kinase (MAPK) were investigated in MSU crystal-induced rat. The levels of SDF-1 and mitogen-activated kinase kinase (MKK) 3/6 were measured by Western Blot in interleukin-1β (IL-1β) incubated fibroblast-like synoviocytes (FLS). A significant increase in the levels of SDF-1, CXCR 4 and p38 MAPK were observed in MSU crystal-induced rat. The increased SDF-1 and MKK 3/6 levels were observed in IL-1β incubated FLS. With the treatment of RDN, the above changes were reverted back to near normal levels. RDN might have some therapeutic effects on GA through SDF-1/CXCR 4 and p38 MAPK pathway, and dioscin may be the active compound in RDN to exert therapeutic effect on GA.
穿山龙（RDN）是一种广泛使用的传统中药，用于治疗关节痛、关节痛和关节炎。众所周知，炎症机制在痛风性关节炎（GA）的发生、病程和预后中起着重要作用。本研究旨在确定 GA 大鼠滑膜和滑膜细胞中表达的特征性蛋白。通过尿酸单钠（MSU）晶体诱导大鼠 GA 模型。采用免疫组织化学方法检测组织样本。研究 RDN 对 MSU 晶体诱导的大鼠基质细胞衍生因子 1（SDF-1）、CXCR4 和 p38 丝裂原活化蛋白激酶（MAPK）的影响。采用 Western blot 法检测白细胞介素-1β（IL-1β）孵育的成纤维样滑膜细胞（FLS）中 SDF-1 和丝裂原激活激酶激酶（MKK）3/6 的水平。在 MSU 晶体诱导的大鼠中，SDF-1、CXCR4 和 p38 MAPK 的水平显著升高。在 IL-1β 孵育的 FLS 中观察到 SDF-1 和 MKK 3/6 水平升高。用 RDN 治疗后，上述变化恢复到接近正常水平。RDN 可能通过 SDF-1/CXCR4 和 p38 MAPK 途径对 GA 具有一定的治疗作用，薯蓣皂苷元可能是 RDN 发挥 GA 治疗作用的活性化合物。
