Kintz P, Mangin P, Lugnier A A, Chaumont A J
Institut de Médecine Légale, Strasbourg.
J Toxicol Clin Exp. 1990 Jan-Feb;10(1):15-20.
A method for identification and quantification of methadone and its primary metabolite (1,5-dimethyl-3,3-diphenyl-2-2-ethylidene pyrrolidine) in plasma by GC/NPD is presented. The procedure employs SKF 525 A as the internal standard and requires no derivatization. After a single-step extraction, analysis is achieved in 10 min. The limit of detection was found to be 2 an 3 ng/ml for methadone and its metabolite, respectively. This method appears to be rapid, sensitive and applicable to forensic and clinical toxicological analyses.
本文介绍了一种采用气相色谱/氮磷检测器（GC/NPD）鉴定和定量血浆中美沙酮及其主要代谢物（1,5-二甲基-3,3-二苯基-2-亚乙基吡咯烷）的方法。该方法使用SKF 525 A作为内标，无需衍生化。经过一步萃取后，10分钟内即可完成分析。美沙酮及其代谢物的检测限分别为2 ng/ml和3 ng/ml。该方法快速、灵敏，适用于法医和临床毒理学分析。
