Analytical Chemistry Department, Faculty of Sciences, University of Burgos, Plaza Misael Bañuelos s/n, 09001 Burgos, Spain.
Talanta. 2013 Jul 15;111:8-12. doi: 10.1016/j.talanta.2013.03.042. Epub 2013 Mar 22.
The current paper presents the chronoamperometric determination of codeine using screen-printed carbon electrodes that incorporate tetrathiafulvalene in the matrix of the working electrode, as mediator, and cross-linked acetylcholinesterase. Applying a potential of +250 mV, a 1mM solution of acetylthiocholine in electrolyte solution pH 7 gives an oxidation signal due to the dimerization of its metabolite after the reaction with the enzyme. This electrochemical signal is decreased by consecutive additions of a solution of codeine, which allows the performance of curves of calibration for the validation of this electrochemical method, giving a reproducibility of 3.31% (n=6) and a capability of detection of 20 µM. This type of inhibition has been studied by means of a Lineweaver-Burk plot. Additionally, the developed biosensor was used for the determination of the quantity of codeine in pharmaceutical commercial tablets and urine samples.
本文提出了一种使用丝网印刷碳电极的计时安培法测定可待因的方法，该电极将四硫富瓦烯作为工作电极的基质中的介体，并交联乙酰胆碱酯酶。在施加+250 mV 的电位下，在电解质溶液 pH 7 中 1mM 的乙酰硫代胆碱溶液由于其代谢物与酶反应后的二聚化而产生氧化信号。该电化学信号通过连续添加可待因溶液而降低，这允许为验证这种电化学方法进行校准曲线的性能，得到 3.31%（n=6）的重现性和 20µM 的检测能力。通过 Lineweaver-Burk 图研究了这种抑制类型。此外，所开发的生物传感器用于测定商业片剂和尿液样品中可待因的含量。
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