Shih Y, Zen J-M, Yang H-H
Department of Applied Cosmetology, Hung-Kuang Institute of Technology, 34 Chung-Chie Rd. Sha-Lu, 433, Taichung, Taiwan, ROC.
J Pharm Biomed Anal. 2002 Jul 31;29(5):827-33. doi: 10.1016/s0731-7085(02)00179-6.
Both flow-injection analysis and square-wave stripping voltammetry were evaluated for the determination of codeine in pharmaceutical formulations using a nontronite clay-modified screen-printed carbon electrode. Compared with a bare screen-printed carbon electrode, the nontronite clay-modified screen-printed carbon electrode exhibited a marked enhancement of the current response of codeine. A linear calibration plot was obtained over the 2.5-45 microM range (correlation coefficient=0.999) in pH 6.0 phosphate buffer solution with a detection limit of 20 nM (S/N=3) by square-wave voltammetry (SWV). While, in flow-injection analysis, the linearity was over 5-120 ng range with a detection limit of 1 ng in 20 microl loop. The nontronite clay-modified screen-printed carbon electrode can be either disposable or reused since the renewal gave a good reproducible surface. Quantitative analysis was performed by the standard addition method for codeine content in both urine and commercially available drugs.
使用绿脱石粘土修饰的丝网印刷碳电极，对流动注射分析和方波溶出伏安法测定药物制剂中可待因的性能进行了评估。与裸丝网印刷碳电极相比，绿脱石粘土修饰的丝网印刷碳电极对可待因的电流响应有显著增强。在pH 6.0的磷酸盐缓冲溶液中，通过方波伏安法（SWV）在2.5 - 45 microM范围内获得了线性校准曲线（相关系数 = 0.999），检测限为20 nM（S/N = 3）。而在流动注射分析中，线性范围为5 - 120 ng，在20微升进样环中的检测限为1 ng。绿脱石粘土修饰的丝网印刷碳电极既可以一次性使用，也可以重复使用，因为表面更新后具有良好的重现性。采用标准加入法对尿液和市售药物中的可待因含量进行了定量分析。
