Department of Chemistry and Biochemistry, California State University, 5151 State University Drive, Los Angeles, CA 90032 8202, USA.
Anal Chim Acta. 2013 May 13;777:72-7. doi: 10.1016/j.aca.2013.03.035. Epub 2013 Mar 22.
Surface plasmon resonance (SPR) spectroscopy is utilized to study in real-time and, by label-free means, the reversible and quasi-irreversible adsorption of small ionic or neutral molecules, pharmaceuticals, and proteins on poly(dimethylsiloxane) (PDMS) surfaces. The SPR sensor is covered with 0.2% (w/v) PDMS in octane. During the timescale of a typical lab-on-a-chip analysis or an electrophoretic separation, it was found that small neutral components containing a hydrophobic part do not adsorb or absorb onto PDMS, while larger, water-soluble polymer-like materials like proteins generally irreversibly adsorb to PDMS. The technique can be used to monitor the kinetics of adsorption and desorption of the molecules. For the non-specific adsorption of teicoplanin to PDMS, a Langmuir-like adsorption isotherm was obtained (K(d)=32±2 μmol L(-1)).
表面等离子体共振（SPR）光谱学被用于实时研究，通过无标记的方式，研究小分子离子或中性分子、药物和蛋白质在聚二甲基硅氧烷（PDMS）表面上的可逆和准不可逆吸附。SPR 传感器表面覆盖有 0.2%（w/v）的辛烷中的 PDMS。在典型的微流控分析或电泳分离的时间尺度内，发现含有疏水部分的小中性成分不会吸附或吸收到 PDMS 上，而像蛋白质这样较大的、水溶性聚合物样的材料通常会不可逆地吸附到 PDMS 上。该技术可用于监测分子的吸附和解吸动力学。对于替考拉宁对 PDMS 的非特异性吸附，得到了类似于朗缪尔的吸附等温线（K(d)=32±2 μmol L(-1)）。
