Nagy Cynthia, Szabo Ruben, Gaspar Attila
Department of Inorganic and Analytical Chemistry, University of Debrecen, Egyetem ter 1., 4032 Debrecen, Hungary.
Micromachines (Basel). 2022 Feb 17;13(2):311. doi: 10.3390/mi13020311.
Given the strong interdisciplinary nature of microfluidic immobilized enzyme reactor (μ-IMER) technology, several branches of science contribute to its successful implementation. A combination of physical, chemical knowledge and engineering skills is often required. The development and application of μ-IMERs in the proteomic community are experiencing increasing importance due to their attractive features of enzyme reusability, shorter digestion times, the ability to handle minute volumes of sample and the prospect of on-line integration into analytical workflows. The aim of this review is to give an account of the current (2017-2021) trends regarding the preparation of microdevices, immobilization strategies, and IMER configurations. The different aspects of microfabrication (designs, fabrication technologies and detectors) and enzyme immobilization (empty and packed channels, and monolithic supports) are surveyed focusing on μ-IMERs developed for proteomic analysis. Based on the advantages and limitations of the published approaches and the different applications, a probable perspective is given.
鉴于微流控固定化酶反应器（μ-IMER）技术具有很强的跨学科性质，多个科学分支都对其成功应用做出了贡献。通常需要物理、化学知识和工程技能的结合。由于μ-IMER具有酶可重复使用、消化时间短、能够处理微量样品以及有望在线集成到分析工作流程等吸引人的特点，它在蛋白质组学领域的开发和应用正变得越来越重要。本综述的目的是阐述当前（2017 - 2021年）关于微器件制备、固定化策略和IMER配置的趋势。针对用于蛋白质组学分析的μ-IMER，对微制造的不同方面（设计、制造技术和检测器）以及酶固定化（空通道和填充通道，以及整体支撑体）进行了综述，并基于已发表方法的优缺点和不同应用给出了可能的前景展望。
