National Research Council Canada, 6100 Royalmount Avenue, Montreal, Quebec H4P 2R2, Canada.
Anal Chim Acta. 2013 May 13;777:78-85. doi: 10.1016/j.aca.2013.03.044. Epub 2013 Mar 26.
Titanium dioxide (TiO2) nanoparticles (NPs) with different sizes and structures were probed for plausible cytotoxicity using electric cell-substrate impedance sensing (ECIS), a non-invasive and on-line procedure for continuous monitoring of cytotoxicity. For insect cells (Spodoptera frugiperda Sf9), the ECIS50 values, i.e., the concentration required to achieve 50% inhibition of the response, differed depending on the size and shape of the TiO2 nanostructure. The lowest ECIS50 value (158 ppm) was observed for the needle shaped rutile TiO2 (10 nm×40 nm, 15.5 nm nominal particle size), followed by 211 ppm for P-25 (34.1 nm, 80% anatase and 20% rutile), 302 ppm for MTI5 (5.9 nm, 99% anatase) and 417 ppm for Hombitan LW-S bulk TiO2 (169.5 nm, 99% anatase). Exposure of TiO2 NPs to UV light at 254 nm or 365 nm exhibited no significant effect on the ECIS50 value due to the aggregation of TiO2 NPs with diminishing photocatalytic activities. Chinese hamster lung fibroblast V79 cells, exhibited no significant cytotoxicity/inhibition up to 400 ppm with P25, MTI5 and bulk TiO2. However, a noticeable inhibitory effect was observed (ECIS50 value of 251 ppm) with rutile TiO2 as cell spreading on the electrode surface was prevented.
采用细胞-基质阻抗感应（ECIS）技术，对不同粒径和结构的二氧化钛（TiO2）纳米颗粒（NPs）进行了潜在细胞毒性的探测，这是一种用于连续监测细胞毒性的非侵入性和在线程序。对于昆虫细胞（草地贪夜蛾 Sf9），ECIS50 值（即达到反应抑制 50%所需的浓度）取决于 TiO2 纳米结构的尺寸和形状。针状锐钛矿 TiO2（10nm×40nm，15.5nm 名义粒径）的 ECIS50 值最低（158ppm），其次是 P-25（34.1nm，80%锐钛矿和 20%金红石）为 211ppm，MTI5（5.9nm，99%锐钛矿）为 302ppm，Hombitan LW-S 块状 TiO2（169.5nm，99%锐钛矿）为 417ppm。暴露于 254nm 或 365nm 的紫外光下的 TiO2 NPs 由于 TiO2 NPs 的聚集而导致光催化活性降低，对 ECIS50 值没有显著影响。在 400ppm 时，P25、MTI5 和块状 TiO2 对中国仓鼠肺成纤维细胞 V79 没有明显的细胞毒性/抑制作用。然而，在电极表面上观察到了明显的抑制作用（ECIS50 值为 251ppm），因为阻止了细胞在电极表面上的扩展。
