CBET Res. Ctr. Experimental Marine Biology and Biotechnology of Plentzia (PIE-UPV/EHU) & Zoology & Cell Biology Dept. (Science and Technology Fac.), University of the Basque Country (UPV/EHU), Bilbao, Spain.
Mar Pollut Bull. 2013 Jun 15;71(1-2):230-9. doi: 10.1016/j.marpolbul.2013.02.034. Epub 2013 Apr 25.
Transcriptional profiling can elucidate adaptive/toxicity pathways participating in achieving homeostasis or leading to pathogenesis in marine biota exposed to chemical substances. With the aim of analyzing transcriptional responses in the mussel Mytilus edulis exposed to the corrosive and putatively carcinogenic hydrocarbon styrene (3-5 ppm, 3days), a forward subtracted (SSH) cDNA library was produced. Female mussels were selected and digestive gland mRNA was isolated. A library with 1440 clones was produced and a total of 287 clones were sequenced, 53% being identified through BlastN analysis against Mytibase and DeepSeaVent databases. Those genes included GO terms such as 'response to drugs', 'immune defense' and 'cell proliferation'. Furthermore, sequences related to chitin and beta-1-3-glucan metabolism were also up-regulated by styrene. Many of the obtained sequences could not be annotated constituting new mussel sequences. In conclusion, this SSH study reveals novel sequences useful to generate molecular biomarkers of styrene exposure in mussels.
转录谱分析可以阐明海洋生物暴露于化学物质时参与维持体内平衡或导致发病机制的适应性/毒性途径。本研究旨在分析贻贝 Mytilus edulis 暴露于腐蚀性和潜在致癌性碳氢化合物苯乙烯（3-5ppm，3 天）后的转录反应，构建了正向消减（SSH）cDNA 文库。选择雌性贻贝并分离消化腺 mRNA。构建了一个包含 1440 个克隆的文库，并对总共 287 个克隆进行了测序，通过 BlastN 分析与 Mytibase 和 DeepSeaVent 数据库的比对，确定了 53%的序列。这些基因包括 GO 术语，如“对药物的反应”、“免疫防御”和“细胞增殖”。此外，苯乙烯还上调了与几丁质和β-1-3-葡聚糖代谢相关的序列。许多获得的序列无法注释，构成了贻贝的新序列。总之，这项 SSH 研究揭示了贻贝中苯乙烯暴露的分子生物标志物生成的新序列。
