Laboratory of Cell Biology and Histology, Faculty of Science and Technology, University of the Basque Country UPV/EHU, Sarriena z/g, E-48940 Leioa, Basque Country, Spain.
Ecotoxicology. 2012 Apr;21(3):820-31. doi: 10.1007/s10646-011-0843-6. Epub 2012 Feb 4.
Recent spills in European waters have released polycyclic aromatic hydrocarbons, important components of heavy fuel oil, and the hydrocarbon styrene. Heavy fuel oil and styrene are classified as potentially genotoxic and carcinogenic. Here we investigate transcription of genes involved in cancer development in the liver of juvenile turbots and in the digestive gland of mussels exposed to heavy fuel oil and to styrene and after a recovery period. In turbot, oil produced a significant up-regulation of p53 and gadd45α after 14 days exposure. cyclin G1 was up-regulated after 7 days treatment with styrene. In mussels, ras was down-regulated in both treatments after the recovery periods. No mutations in ras hotspots were detected in exposed mussels. gadd45α was up-regulated after the recovery period of the styrene experiment. Overall, transcriptional responses differed in mussels compared to turbot. Turbot responded to hydrocarbon exposure by triggering cell cycle arrest (p53) and DNA repair (gadd45α).
最近在欧洲水域发生的溢油事件释放了多环芳烃，这是重质燃料油和碳氢化合物苯乙烯的重要成分。重质燃料油和苯乙烯被归类为具有潜在遗传毒性和致癌性的物质。在这里，我们研究了暴露于重质燃料油和苯乙烯以及恢复期后，幼比目鱼肝脏和贻贝消化腺中与癌症发展相关的基因的转录。在比目鱼中，油在暴露 14 天后显著上调了 p53 和 gadd45α。在苯乙烯处理 7 天后，细胞周期蛋白 G1 上调。在贻贝中，两种处理后 ras 在恢复期都下调。在暴露的贻贝中未检测到 ras 热点突变。在苯乙烯实验的恢复期后，gadd45α 上调。总体而言，贻贝和比目鱼的转录反应不同。比目鱼通过触发细胞周期停滞（p53）和 DNA 修复（gadd45α）来应对烃类物质的暴露。
