Key Laboratory of Industrial Ecology and Environmental Engineering (MOE), School of Environmental Science and Technology, Dalian University of Technology, Linggong Road 2, Dalian 116024, PR China.
Bioresour Technol. 2013 Jun;138:339-44. doi: 10.1016/j.biortech.2013.03.181. Epub 2013 Apr 4.
The nitrate- and Fe(III)-reducing bacterium Paracoccus versutus LYM was characterized in terms of its ability to perform Fe(II)EDTA-NO reduction coupled with Fe(II)EDTA oxidation (NO-dependent Fe(II)EDTA oxidation, NDFO). It experienced a single anaerobic FeEDTA redox cycling through NDFO and dissimilatory Fe(III)EDTA reduction in FeEDTA culture. The increase in the Fe(II)EDTA concentration contributed to the ascending Fe(II)EDTA-NO reduction rate. The amount of glucose controlled the rate and extent of Fe(II) oxidation during NDFO. Without glucose addition, Fe(II)EDTA-NO reduction rate was at a rather slow rate even in presence of relatively sufficient Fe(II)EDTA. Unlike aqueous Fe(2+) and solid-phase Fe(II), Fe(II)EDTA could prevent cells from encrustations. These findings suggested the occurrence of NDFO preferred being beneficial via a mixotrophic physiology in the presence of an organic cosubstrate to being out of consideration for metabolic strategy.
硝酸盐和 Fe(III)还原菌 Paracoccus versutus LYM 的特性在于其能够进行 Fe(II)EDTA-NO 还原与 Fe(II)EDTA 氧化偶联（NO 依赖的 Fe(II)EDTA 氧化，NDFO）。它通过 NDFO 和异化 Fe(III)EDTA 还原在 FeEDTA 培养物中经历了单一的厌氧 FeEDTA 氧化还原循环。Fe(II)EDTA 浓度的增加促进了 Fe(II)EDTA-NO 还原速率的上升。葡萄糖的量控制着 NDFO 过程中 Fe(II)氧化的速率和程度。如果不添加葡萄糖，即使存在相对充足的 Fe(II)EDTA，Fe(II)EDTA-NO 还原速率也相当缓慢。与水合 Fe(2+)和固相 Fe(II)不同，Fe(II)EDTA 可以防止细胞结垢。这些发现表明，在存在有机共底物的情况下，通过混合营养生理学发生 NDFO 更倾向于有利，而不是出于代谢策略的考虑。
